高压氧对局灶性脑缺血再灌注大鼠环加氧酶基因表达及血脑屏障通透性的影响  

Effects of Hyperbaric Oxygen on Expression of Cyclooxygenase in the Brain Tissues and Blood Brain Barrier after Local Cerebral Ischemia-reperfusion in Rats

作　　者：赵红[1] 张倩茹[1] 佟雪[2] 陈学新[3] 

机构地区：[1]中国医科大学基础医学院机能实验中心,沈阳110001 [2]沈阳医学院 [3]中国医科大学第一临床医院高压氧科,沈阳110001

出　　处：《中国康复》2011年第2期86-88,共3页Chinese Journal of Rehabilitation

基　　金：辽宁省教育厅高校科研基金资助项目(2005L456);辽宁省科技厅博士启动基金资助项目(20101149)

摘　　要：目的:探讨高压氧(HBO)对大鼠局灶性脑缺血再灌注环加氧酶-1(COX-1)、环加氧酶-2(COX-2)mRNA的表达及血脑屏障(BBB)通透性的影响。方法:160只Wister大鼠随机分为A、B、C、D 4组各40只。成功制备为局灶性脑缺血再灌注大鼠模型,术后C、D组于2、9、11、45及69 h点均行0.25 MPa HBO治疗。采用逆转录多聚酶链反应(RT-PCR)法及比色法分别检测大鼠4、11、23、48及72 h点脑组织中COX-1、COX-2 mRNA的表达及伊文思蓝(EB)的浓度。结果:与A、C组比较,B、D组各时间点COX-1 mRNA的表达差异无统计学意义;而COX-2 mRNA的表达及EB的浓度在11～72 h点组明显高于A、C组(P<0.05,0.01)。结论:HBO具有降低脑缺血再灌注过程中COX-2 mRNA的表达,对血脑屏障具有保护作用,而对COX-1 mRNA的表达作用不明显。Objective： To investigate the effects of hyperbaric oxygen（HBO） on expressions of cyclooxygenase（COX） in the brain tissues and blood brain barrier（BBB） after local cerebral ischemia-reperfusion in rats.Methods： 480 Wistar rats were randomly divided into 4 groups： sham-operation group,cerebral ischemia-reperfusion（IR） group,HBO group,HBO＋cerebral ischemia-reperfusion（HBO＋IR） group.The model of focal cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion（MCAO）,0.25 MPa（ATA） HBO was applied 5 times during the reperfusion period,and 2% Evens blue（EB） was injected into tail veins one h before the animals were killed.The expression of COX mRNA and the contents of EB were determined by RT-PCR and spectrophotometer.Results： There was no significant difference in the expression of COX-1 mRNA between IR group and sham-operation group.COX-2 mRNA expression level and the contents of EB were significantly increased in IR group as compared with the sham-operation group（P0.05）.The expression levels of COX-1 and COX-2 mRNA and the contents of EB in HBO group were similar to those in the sham-operation group.The expression of COX-2 mRNA and the contents of EB group were significantly decreased as compared with IR group（P0.01）,but there was no significant difference in the expression level of COX-1 mRNA between HBO＋IR group and IR group.Conclusion： HBO can reduce the expression of COX-2 mRNA and protect the permeability of BBB during cerebral ischemia-reperfusion,but has no obvious effects on the COX-1 mRNA expression.

关 键 词：高压氧 血脑屏障 缺血再灌注 环加氧酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]