蜕皮甾酮对高糖培养大鼠肾小球系膜细胞增殖及其细胞外基质含量的影响  

EFFECTS OF ECDYSTERONE ON PROLIFERATION OF MESANGIAL CELLS AND CONCENTRATION OF EXTRACELLULAR MATRIX IN HIGH GLUCOSE CULTURED RAT MESANGIAL CELLS

作　　者：高岑[1] 马跃荣[1] 史书龙[1] 陈秋[2] 

机构地区：[1]泸州医学院病理教研室,四川泸州646000 [2]成都中医药大学附属医院内分泌科

出　　处：《泸州医学院学报》2011年第4期330-333,共4页Journal of Luzhou Medical College

基　　金：四川省重大攻关及应用基础项目(2006J13-033)

摘　　要：目的:观察蜕皮甾酮对高糖环境下大鼠肾小球系膜细胞(MCs)增殖、细胞外基质的影响。方法:体外培养大鼠MCs细胞株,分低糖组、高糖组、高糖+蜕皮甾酮组(EDS组),分别作用12、24、48h,MTT法检测细胞增殖活力,ELISA法检测细胞上清中FN的含量。结果:与低糖组比较,高糖组12h、24h、48h MCs增殖显著增快(P<0.01),FN含量明显增加(P<0.05);与低糖组比较,EDS在浓度>1×10-5mol/L时MCs增殖显著减慢(P<0.01);与高糖组比较,EDS 1×10-6mol/L组各时间点及1×10-7mol/L组24h、48h MCs增殖显著减慢(P<0.05或P<0.01),EDS 1×10-6mol/L组各时间点FN含量均有降低(P<0.05或P<0.01);EDS 1×10-10、1×10-8mol/L组各时间点FN含量差异无统计学意义(P>0.05)。结论:①蜕皮甾酮在浓度>1×10-5mol/L时对MCs有明显的细胞毒性作用。②蜕皮甾酮可抑制高糖诱导的MCs增殖。③蜕皮甾酮能减少高糖培养大鼠MCs细胞外基质的增加。Objective： To investigate the effects of ecdysterone on proliferation of mesangial cells（MCs） and concentration of extracellular matrix in high glucose cultured rat MCs.Methods： Rat MCs were divided into low glucose group,high glucose group and high glucose＋ecdysterone group（EDS group which was subdivided according to different final concentration of ecdysterone）,and were cultured for 12h,24h and 48h.At indicated time points,cellular proliferation was assessed by MTT,the levels of fibronectin in supernatant were assayed by enzyme-linked immunosorbent assay.Results： Compared with low glucose group,the cellular proliferation and contents of FN increased significantly in high glucose group at 12h,24h and 48h（P0.01）.In the cellular OD value of EDS1×10-5mol/L group MCS proliferation decreased significantly compared with low glucose group（P0.01）.Compared with high glucose group,the cellular proliferation decreased significantly in EDS 1×10-6mol/L group at every time point and 1×10-7mol/L group at 24h,48h（P0.05 or P0.01）,the concentration of FN declined in EDS 1×10-6mol/L group（P0.01 or P0.05）,while there was no significant differences between EDS 1×10-10 mol/L group and 1×10-8mol/L group（P0.05）.Conclusion：①For MCs,ecdysterone may have evident cytotoxicity when its concentration 1×10-5mol/L.②Ecdysterone can significantly inhibit proliferation of high glucose induced rat mesangial cells.③Ecdysterone can significantly inhibit secretion of extracellular matrix in high glucose induced rat mesangial cells.

关 键 词：蜕皮甾酮 糖尿病肾病 肾小球系膜细胞 细胞外基质 

分 类 号：R365[医药卫生—病理学] E587.1[医药卫生—基础医学]