古抑菌素A对肝癌细胞HepG2中抑癌基因FHIT表达的影响  

EFFECT OF TRICHOSTATIN A ON FHIT GENE EXPRESSION IN HUMAN HEPATOMA CELL LINE HEPG2

作　　者：王丽[1] 段承刚[1] 梅志强[1] 甘淋[1] 何涛[1] 

机构地区：[1]泸州医学院医学基础研究中心,四川泸州646000

出　　处：《泸州医学院学报》2011年第4期352-355,共4页Journal of Luzhou Medical College

基　　金：四川省卫生厅科研资助项目(060065)

摘　　要：目的:探讨去乙酰化转移酶抑制剂古抑菌素A(trichostatin A,TSA)对人肝癌HepG2细胞生长的作用以及FHIT表达的影响。方法:体外培养人肝癌细胞株HepG2,分为对照组和不同浓度(5、50、250、500、1000nmol/L)TSA组,作用24h和48h后在倒置显微镜下观察细胞形态改变,CCK-8法检测TSA对HepG2细胞增殖的影响,实时荧光定量RT-PCR法检测FHIT基因的表达。结果:倒置显微镜下,经TSA处理的细胞增殖速度显著减慢,出现凋亡早期改变,与对照组相比,TSA可明显抑制HepG2的增殖,且呈时效和剂量依赖关系(P<0.01),荧光定量PCR结果显示TSA可以使FHIT表达上调(P<0.01)。结论:TSA对肝癌HepG2细胞体外生长有明显抑制作用,可能与促进FHIT表达有一定关系。Objective： To investigate the growth-inhibitory effect of trichostatin A（TSA） on proliferation and expression of fragile histidine triad（FHIT） in human hepatoma cell strain HepG2.Methods： Cultured cells were divided into control group and TSA group,and treated with DMSO and different concentration of TSA（5,50,250,500,1 000 nmol/L） respectively.The cells were collected 24 and 48 hours later and cell morphology was observed by inverted light microscope.Then the effect of TSA on cell activity was observed by CCK8 and the expression of FHIT was analyzed by using real-time RT-PCR.Results： Under inverted light microscope,the early apoptosis of HepG2 cells was observed and the proliferation of HepG2 cell was inhibited significantly after treatment of TSA.Compared with control group,TSA inhibited the proliferation of HepG2 cells in dose-dependent and time-dependent manners（P0.01）.The expression of FHIT mRNA was increased（P0.01）.Conclusion： TSA can inhibit the growth of hepatocarcinoma HepG2 cells,which may be associated with up-regulating the expression of FHIT gene.

关 键 词：脆性组氨酸三联体 古抑菌素A 肝癌 实时荧光定量RT-PCR 

分 类 号：R735.7[医药卫生—肿瘤] Q503[医药卫生—临床医学]