骨髓间充质干细胞修复损伤脊髓的实验及临床应用短期随访  

Experimental study and short-term clinical follow-up of bone mesenchymal stem cells transplantation for repair of spinal cord injury

作　　者：高德萱[1] 贾全章[2] 李东君[1] 陈玉丙[3] 孙景海[2] 王风华[2] 徐爽[2] 刘丽萍[3] 姜大伟[1] 

机构地区：[1]延边大学医学部,吉林省延吉市133002 [2]解放军第208医院,吉林省长春市130062 [3]吉林大学附属第二医院,吉林省长春市130000

出　　处：《中国组织工程研究与临床康复》2011年第27期5016-5020,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：吉林省科技发展计划项目(20080446)"骨髓间充质干细胞对脊髓损伤模型大鼠的修复作用及临床应用研究"~~

摘　　要：背景:骨髓间充质干细胞治疗脊髓损伤的研究已经逐渐由动物实验过渡到临床,但其作用机制还不完全清楚。目的:观察骨髓间充质干细胞移植对脊髓损伤大鼠脊髓功能的修复作用,并通过临床应用观察短期疗效。方法:采用改良Allen's打击法造成Wistar大鼠脊髓损伤模型,将体外分离培养的骨髓间充质干细胞分别经尾静脉及损伤局部移植,应用改良Tarlov评分评定大鼠行为学变化,在光镜下对损伤脊髓病理切片进行对比分析。对5例脊髓损伤患者通过损伤原位注射、腰穿、静脉输注的方式行人自体骨髓间充质干细胞移植,并行神经功能及生活能力评定。结果与结论:治疗后15d,骨髓间充质干细胞尾静脉及损伤局部移植组大鼠运动功能评分较模型对照组显著提高;移植后7,15,30d,脊髓病理切片显示骨髓间充质干细胞尾静脉及损伤局部移植组大鼠较模型对照组有显著恢复。临床患者骨髓间充质干细胞移植后半年神经功能及生活能力均有改善。BACKGROUND：Studies of bone marrow mesenchymal stem cells（BMSCs） have gradually experienced a transition from animal experiments to clinical application.However,the mechanism is still unclear.OBJECTIVE：To observe the repairing effect and short-term effect of BMSCs transplantation on rats motor function.METHODS：Spinal cord injury models were prepared by modified Allen＇s crack method;the BMSCs which were isolated and cultured in vitro were transplanted into the model through the vena caudalis and the local lesion.The behavioral changes were observed using the modified Tarlov score standard and the pathological changes under the light microscope.Five patients with spinal cord injury were transplanted autologous BMSCs via in suit injection,lumbar puncture and venous injection.Neurologic function and ability of daily living were evaluated.RESULTS AND CONCLUSION：After 15 days,there was a significant increase in the score of motion function of rats receiving BMSCs transplantation as compared with the control group.Through observing the spinal pathological section after transplantation,there was significantly more recovery in the BMSCs group than the model group at post-transplantation 7,15,30 days.After 6 months of short-term observation,the neurologic function and ability of daily living were improved in patients undergoing BMSCs transplantation.

关 键 词：脊髓损伤 骨髓间充质干细胞 修复 神经功能 生活能力 

分 类 号：R394.2[医药卫生—医学遗传学]