骨髓间充质干细胞尾静脉移植脊髓损伤大鼠脑源性神经营养因子及神经生长因子的表达  

Influence of tail vein administration of bone marrow mesenchymal stem cells on expression of brain-derived neurotrophic factor and nerve growth factor after spinal cord injury in rats

作　　者：董锋[1] 林建华[1] 吴朝阳[1] 

机构地区：[1]福建医科大学附属第一医院骨科,福建省福州市350005

出　　处：《中国组织工程研究与临床康复》2011年第27期5026-5030,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：福建省科技厅重点项目(2002Y014);福州市科技局项目(2002-14)~~

摘　　要：背景:骨髓间充质干细胞移植对脊髓损伤有治疗作用,但其机制尚不完全清楚。目的:应用免疫组织化学方法观察骨髓间充质干细胞静脉移植损伤脊髓局部脑源性神经营养因子及神经生长因子的表达,分析骨髓间充质干细胞移植治疗大鼠脊髓损伤的作用途径。方法:运用改良Allen法制备T10脊髓外伤性截瘫大鼠模型,假手术组6只,脊髓损伤组24只随机分为对照组和骨髓间充质干细胞移植组。骨髓间充质干细胞移植组、假手术组接受骨髓间充质干细胞单细胞悬液1mL(1×106cells)自大鼠尾静脉缓慢注射移植,对照组静脉注射PBS1mL。结果与结论:脊髓损伤后损伤局部的脑源性神经营养因子、神经生长因子表达增加,骨髓间充质干细胞静脉注射移植后能促进脊髓损伤局部脑源性神经营养因子、神经生长因子更进一步的表达,这可能是促进神经结构及神经功能恢复的因素之一。BACKGROUND：Transplantation of bone marrow mesenchymal stem cells has an effect on the treatment of spinal cord injury,but the mechanism is not fully understood.OBJECTIVE：To explore the influence of intravenous administration of bone marrow mesenchymal stem cells on expression of brain-derived neurotrophic factor（BDNF） and nerve growth factor（NGF） after spinal cord injury in rats and to study possible mechanism of intravenous administration of bone marrow mesenchymal stem cells after spinal cord injury in adult rats.METHODS：Modified Allen method was applied to induce T10 spinal cord injury to prepare rat models of paraplegia.There were 6 rats in sham operation group and 24 rats in injury group.Then,the injury group was randomly divided into transplantation group and control group.Rats in the sham operation and transplantation groups received tail vein transplantation of 1 mL bone marrow mesenchymal stem cells（1×106 cells）,while those in the control group were treated with PBS.RESULTS AND CONCLUSION：After spinal cord injury in rats,the expression of BDNF and NGF was improved and advanced to increase via intravenous administration of bone marrow mesenchymal stem cells.Maybe it is one of the mechanisms of promoting restoration of injured spinal cord and improving motor function.

关 键 词：脑源性神经营养因子 脊髓损伤 骨髓间充质干细胞 神经生长因子 细胞移植 

分 类 号：R394.2[医药卫生—医学遗传学]