软骨损伤后基质细胞衍生因子1的表达  

Expression of stromal cell-derived factor-1 after articular cartilage injury

作　　者：陆定贵[1] 韦庆军[1] 李桥川[2] 韦积华[1] 肖仕辉[1] 李伟岸[1] 

机构地区：[1]广西医科大学第一附属医院创伤骨科手外科,广西壮族自治区南宁市530021 [2]广西医科大学第一附属医院干细胞移植中心,广西壮族自治区南宁市530021

出　　处：《中国组织工程研究与临床康复》2011年第27期5063-5067,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广西留学回国人员科学基金(305696)资助~~

摘　　要：背景:研究表明基质细胞衍生因子1对骨髓间充质干细胞的迁移、聚集有影响。目的:观察软骨损伤后不同时间损伤修复区组织基质细胞衍生因子1的表达以及与骨髓间充质干细胞迁移的关系。方法:建立兔软骨损伤模型,分别于建模后2,5,7,10,14,28d取损伤灶及边缘区组织,检测基质细胞衍生因子1表达和体外细胞迁移实验,观察基质细胞衍生因子1对骨髓间充质干细胞、软骨细胞的迁移影响。结果与结论:基质细胞衍生因子1的表达呈现出时间变化的趋势,软骨损伤后第7天达到高峰(P<0.05)。体内移植的骨髓间充质干细胞主要聚集在软骨损伤灶周围,但在封闭CXC趋化因子受体4后,此聚集现象逐渐减弱(P<0.05)。结果证实,局部组织中基质细胞衍生因子1的表达在软骨损伤早期明显升高,对骨髓间充质干细胞向软骨损伤修复区迁移有重要作用。BACKGROUND：Studies have demonstrated that stromal cell-derived factor-1（SDF-1） can influence the migration and aggregation of bone marrow mesenchymal stem cells（BMSCs）.OBJECTIVE：To observe the expression of SDF-1 at lesion regions at different time after cartilage injury and to investigate the correlation between SDF-1 expression and BMSCs migration.METHODS：Rabbits models of articular cartilage injury were established.Focal osteochondral lesions were collected 2,5,7,10,14 and 28 days after injury.The influence of SDF-1 expression on the migration of BMSCs and chondrocytes was observed.RESULTS AND CONCLUSION：SDF-1 expression was significantly increased and peaked at the seventh day（P〈0.05）.BMSCs migrated toward articular cartilage injury tissue,while they were significantly inhibited by the inhibitor of CXCL4.SDF-1 expression increases in the early phase after articular cartilage injury,and plays an important role in chemoattracting MSCs to injured lesions.

关 键 词：基质细胞衍生因子1 骨髓间充质干细胞 CXC趋化因子受体4 软骨损伤 组织工程 

分 类 号：R394.2[医药卫生—医学遗传学]