牛奶中尿素现场快速检测方法的研究  

Study of Field Rapid Determination Method of Urea in Milk

作　　者：宋丹[1] 朴仁官 丁毅[1] 郭蔷薇[1] 刘静[3] 高德江[3] 于爱民[1,3] 

机构地区：[1]吉林大学化学学院,长春130012 [2]长春光学精密机械与物理研究所,长春130033 [3]长春吉大.小天鹅仪器有限公司,长春130012

出　　处：《现代科学仪器》2011年第4期35-38,共4页Modern Scientific Instruments

基　　金：吉林省科技发展计划项目经费资助(20096016)

摘　　要：本文建立了一种现场快速检测牛奶中尿素含量的方法。系统考察了试剂用量、反应时间和温度等因素对测定结果的影响。结果表明,在尿素含量为0.001%～0.050%范围内,尿素浓度与反应体系吸光度间具有良好的线性关系,其线性相关系数R=0.9998;分别对高、中、低3个浓度的样品,进行了6次重复测定,相对标准偏差在1.32%～2.04%之间。在三个不同浓度添加水平下,加标回收率为99%～105%。从实验结果可知该方法具有较高的灵敏度和准确度,而且操作简单,整个检测过程仅需10 min,适用于牛奶中尿素含量的现场快速定量检测,为生鲜乳安全提供有效的检测手段。A field rapid determination method of urea in milk is investigated in this paper.Effects of reagent dosage,reaction time and temperature on determination of urea were studied respectively.Under the optimal conditions,the calibration curves showed a good linearity in the concentration range of 0.001%0.050%,and the coefficient was 0.9998.The relative standard derivation（RSD） for different concentrations was 1.32%2.04%,and the recovery was 99%105%.The experimental results showed that the determination method of urea in this paper has the characteristics of simplicity,rapidity and accuracy,and is suitable for determination of urea in milk

关 键 词：牛奶 尿素 现场 快迷 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]