堆肥过程多阶段强化接种对细菌群落多样性的影响  

Effects of Multi-stage Strengthening Inoculation on Bacterial Community Diversity During Composting

作　　者：党秋玲[1,2] 李鸣晓[2] 席北斗[1] 魏自民[2] 刘驰[1] 夏训峰[1] 杨天学[1] 李晔[2] 

机构地区：[1]中国环境科学研究院水环境系统工程研究室,北京100012 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《环境科学》2011年第9期2689-2695,共7页Environmental Science

基　　金：国家自然科学基金项目(50878201);国家科技支撑计划课题项目(2006BAC06B04;2008BADC4B00;2009BADC2B04);农业科技成果转化资金项目(2008GB24420470)

摘　　要：以生活垃圾为原料进行好氧堆肥,采用多阶段的方式强化接种功能微生物菌剂,利用PCR-DGGE方法并结合聚类分析和Shannon-Weaver指数变化来研究堆肥过程中多阶段强化接种对细菌群落多样性的影响,同时监测木质纤维素降解率的变化.结果表明,多阶段接种堆肥能有效提高堆体降温期和二次发酵期的温度;和普通接种堆肥相比可使半纤维素、纤维素和木质素的降解率分别提高7.19%、10.89%和8.98%.细菌群落多样性分析表明,2种接种方式的多样性指数表现出极显著差异,采用多阶段的方式进行接种能使接种菌剂更好地定植于堆体之中,有效避免不同功能的接种菌剂之间、接种菌剂和土著菌之间的竞争性抑制作用,提高堆体中特别是堆肥腐熟阶段的细菌群落多样性,加快堆肥的稳定化进程.Effects of multi-stage strengthening inoculation on bacterial community diversity were analyzed using PCR-DGGE method in municipal solid waste composting combined with Cluster analysis and changes of Shannon-Weaver index,and the changes of lignocellulose＇s degradation rate were also detected during the process.The results showed that Multi-stage inoculation of composting can improve the temperature of cooling and the secondary fermentation period.And the hemicellulose,cellulose and lignin degradation rate increased by 7.19%,10.89% and 8.98% compared to general inoculation composting.The analysis of bacterial community diversity indicated that the diversity index of the two inoculation showed significant differences,the microbial inoculation could live well in the pile.It could avoid competition between different inoculated agents and competition between inoculated and indigenous microorganisms,and could improve bacterial community diversity during composting especially for maturity stage.It could speed up the composting process of stabilization.

关 键 词：堆肥 多阶段接种 接种菌剂 PCR-DGGE 细菌群落多样性 

分 类 号：X705[环境科学与工程—环境工程]