牛IgG电化学纳米免疫传感器的研制  

Development of Nano-gold-modified Electrochemical Immunosensor for Bovine IgG

作　　者：康晓斌[1] 庞广昌[1] 梁新义[1] 

机构地区：[1]天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津300134

出　　处：《食品科学》2011年第16期379-383,共5页Food Science

基　　金：“十一五”国家科技支撑计划项目(2006BAD04A00)

摘　　要：研制特异性定量检测牛初乳制品及含牛IgG制品中牛IgG含量的电流型纳米免疫传感器。以成膜性及生物相容性良好的壳聚糖为媒介连接纳米金于玻碳电极,以比表面积大、吸附能力强、生物相容性好的纳米金固定兔抗牛IgG-HRP。通过循环伏安法及交流阻抗法表征电极组装各个过程的电化学特性,利用计时电流法测定PBS稀释的标准牛IgG。结果表明免疫前后稳定电流的变化率与标准牛IgG质量浓度的对数在0.1～10000ng/mL范围内线性相关,相关系数r=0.9976。该传感器稳定性及重现性良好,操作简单方便,成本较低,可用于含牛IgG制品中牛IgG含量的特异性定量检测。A current-type nano-immunosensor was developed for the specific determination of immunoglobulin G（IgG） content in colostrum products and bovine IgG-containing products.The chitosan with excellent film-forming capability and biocompatibility was used as the medium to connect nano-gold and glassy carbon electrode.Due to its large surface area,strong adsorption capacity and good biocompatibility,nano-gold was used to immobilize rabbit anti-bovine IgG-HRP.Electrochemical properties of electrode surface during each fabrication procedure were characterized by cyclic voltammetry and alternating current impedance method and standard bovine IgG in phosphate buffer was determined by chronoamperometry.The results revealed that the current-type nano-immuosensor had an excellent linear relationship between the concentration of bovine IgG in the range of 0.1-10000 ng/mL and the change rate of stable current before and after immunization with a correlation coefficient of 0.9976.Moreover,this immunosensor proved stable,reproducible,easy to use and inexpensive,thus being applicable for the quality control of bovine IgG products.

关 键 词：牛免疫球蛋白G(牛IgG) 免疫传感器 纳米金 计时电流法 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.1[轻工技术与工程—食品科学与工程]