体外调节Th1／Th2平衡治疗脑胶质瘤  

Experimental study on adjustment of the immunologic balance between Thl and Th2 for glioma

作　　者：许洪升[1] 孙飞[1] 

机构地区：[1]江苏徐州市中心医院神经外科,221009

出　　处：《中华实验外科杂志》2011年第9期1425-1427,共3页Chinese Journal of Experimental Surgery

基　　金：徐州市科技计划资助项目（X200523111）

摘　　要：目的观察外源性细胞因子白细胞介素（IL）-2、干扰素（IFN）-γ和IL-4单克隆抗体（McAb）、IL-10McAb在体外能否促使Th2型细胞因子分泌优势向Th1型逆转，抑制胶质瘤细胞生长。方法体外培养大鼠C6胶质瘤细胞，加入外源性细胞因子共同孵育，光学显微镜观察细胞形态学变化，噻唑蓝（MIT）比色法检测细胞增殖抑制率，逆转录-聚合酶链反应（RT—PCR）检测Th1／Th2类细胞因子表达变化。结果实验组较对照组细胞体积变小，生长密度低，细胞数目少；实验组与对照组比较细胞增殖抑制率分别为（26．41±3．60）％，其A值与对照组比较差异有统计学意义（P〈0．05）。对照组细胞只表达Th2类细胞因子，Th1类细胞因子表达缺如，实验组IFN-γ表达增加，IL4和IL-10表达减弱，但是未见IL-2表达。结论外源性细胞因子能够抑制脑胶质瘤细胞的生长，促进Th2型细胞因子分泌优势向Th1型逆转。Objective To study whether exgenous interleukin （IL） -2, interferon （IFN）-γ and IL-4 monoclonal antibody （McAb） and IL-10 McAb can cause the superiority of Th2-type cytokines secretion to the Thl reversion and inhibit the growth. Methods In vitro C6 cells were cultured in the presence of exogenous IL-2, IFN-γ, IL-4 McAb and IL-10 McAb. The cell morphology changes were observed under the microscopy. The gene expression of Thl/Th2 cytokines was detected by using reverse transcription-pol- ymerase chain reaction （RT-PCR）, and inhibition rate of cell proliferation was measured by using methyl thiazol tetrazolium （MTT） assay. Results In control group, the cells had irregular morphology, the cells were shrinkaged, the volume, number of ceils and the cells growth density were reduced obviously as compared with experimental group. The inhibition rate of cells proliferation in control group was （26. 41 ± 3.60） % （ P 〈 0. 05 ）. RT-PCR result showed the exprimental group had IFN-γ expression, but had no expression of IL-2 and IL-4, and the expression of IL-10 was relatively weakened. Conclusion The experimental results indicated that exogenous Thl-type cytokines and Th2-type cytokines antibody may inhibit glioma cells growth, and may promote the superiority of Th2-type cytokines secretion to the Thl reversion.

关 键 词：TH1/TH2 免疫调节 胶质瘤 

分 类 号：R739.4[医药卫生—肿瘤]