封闭乳腺癌转移抑制基因1的表达可抑制乳腺癌细胞MCF-7的转移  

Blocking breast cancer metastasis suppressor 1 expression inhibits the metastasis ability of human breast cancer cell line MCF-7

作　　者：高梅娟[1] 田莉[2] 王洪才[3] 

机构地区：[1]北京工业大学医院外科,100124 [2]北京工业大学医院内科,100124 [3]北京工业大学医院中医科,100124

出　　处：《中华实验外科杂志》2011年第9期1459-1461,共3页Chinese Journal of Experimental Surgery

摘　　要：目的构建乳腺癌中乳腺癌转移抑制基因1（BRMS1）反义基因重组腺病毒载体，并观察其对乳腺癌细胞MCF-7转移能力的影响。方法应用基因重组技术将BRMS1反义基因构建于腺病毒载体pAD-X，转染293包装细胞得到高滴度重组腺病毒，并用real-time聚合酶链反应（PCR）进行BRMSl基因表达的验证。将重组腺病毒pAD—aBRMS1感染MCF-7细胞后，应用Tran-_swell小室法观察对细胞侵袭和运动能力的影响。结果酶切结果与预期相符，real-timePCR可检测到感染BRMS1反义基因重组腺病毒后MCF-7细胞没有BRMSl基因表达。病毒转染后Transwell小室可见，MCF-7细胞的侵袭和运动能力均受到显著的抑制（P值均〈0．01）。结论重组反义BRMS1腺病毒载体正确构建，其对乳腺癌细胞MCF-7的侵袭和运动都有抑制作用。Objective To construct breast cancer metastasis suppressor 1 （BRMS1） gene adenovirus vector and observe its effect on the metastasis ability of breast cancer cell line MCF-7. Methods BRMS1 gene was constructed into adenovirus vector pAD-X by gene recombination technique, which was transformed into 293 packaged cells for high titer adenovirus. Real-time polymerase chain reaction （PCR） was applied to detect BRMS1 gene expression. Transwell cabin assay was used to observe changes of inva- sion and motor ability of MCF-7 ceils transfected with recombinant adenovirus. Results The finding of di- gestion was coincided with expected. No BRMS1 gene expression was detected in transfected MCF-7 cells by using real-time PCR. The invasion and motor abilities of transfected MCF-7 cells were significantly inhibited in transweil cabin assay （P 〈0. 01 ）. Conclusion BRMS1 adenovirus was correctly reconstructed and it could inhibit invasion and motor abilities of transfected MCF-7 ceils.

关 键 词：BRMS1 重组腺病毒 乳腺癌 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]