小型猪激光焊接重建硬膜缺损模型  

The minipig model of CO2 laser fascia to dura soldering for dural reconstruction

作　　者：钟红亮[1] 王振民[2] 赵赋[1] 杨智君[1] 刘丕楠[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科北京市神经外科研究所,北京100050 [2]首都医科大学附属北京天坛医院神经外科

出　　处：《中华实验外科杂志》2011年第9期1585-1587,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30870752）

摘　　要：目的探讨建立小型猪激光焊接重建硬膜缺损模型的方法。方法将10头小型猪随机分为两组。额顶部剪除约1cm×2cm硬膜形成缺损，自体颞肌筋膜采用激光焊接进行重建。术后10rain和2周检查焊接组织水密性并测量爆裂压力。结果重建组织均无脑脊液渗出。术后10min和2周的爆裂压力[分别为（25．60±9．13）mmHg（1mmHg=0．133kPa）和（198．60±99．75）mmHg]远高于颅内压峰值（8．40±1．14）mmHg，差异有统计学意义（P〈0．01）。2周组小型猪术后1～2d恢复正常，无感染等并发症。结论小型猪适合建立激光焊接重建硬膜动物模型，手术操作简便，术后动物恢复快，无感染等并发症，重建组织水密性和连接强度好。Objective To establish the minipig model of CO2 laser fascia to dura soldering for du- ral reconstruction. Methods Ten minipigs underwent craniotomy with a creation of a dural defect about 1 cmx 2 cm, and the defect was reconstructed with autologous temporalis fascia flap by CO2 laser soldering. The animals were randomized into 10-min group （ n = 5 ） and 2-week group （ n = 5 ）. Measures for estimation of reconstruction quality included visual assessment under magnification and measurement of burst pressure. Additionally, the breathless intracranial pressure was also measured as a baseline. Results All the 10 minipigs demonstrated no cerebrospinal fluid （CSF） leakage, and the 5 raised minipigs recovered quikly in 1-2 days without any postoperative complications. The burst pressure in 10-rain group and 2-week goup [ （25.60 ± 9. 13 ）mm Hg （ 1 mm Hg =0. 133 kPa） and （ 198. 60 ± 99.75 ） mm Hg, respectively ] was higher than the breathless intracranial pressure [ （8.40 ± 1.14） mm Hg （P 〈0. 01 ）. Conclusion Minipigs can be appropriate animals for CO2 laser fascia to dura soldering for dural reconstruction with quick recovery, easy manipulation and no surgical complications. The bond is watertight and strong.

关 键 词：小型猪 硬膜重建 CO2激光 焊接 

分 类 号：R-332[医药卫生]