利用抗体芯片分析肾间质纤维化的致病因子  

ANTIBODY MICROARRAY ANALYSIS OF PATHOGENIC FACTORS IN RENAL INTERSTITIAL FIBROSIS

作　　者：张旭[1] 张翠薇[1] 高岑[1] 江燕[1] 荣智利[1] 马跃荣[1] 

机构地区：[1]泸州医学院病理教研室,四川泸州646000

出　　处：《泸州医学院学报》2011年第5期505-510,共6页Journal of Luzhou Medical College

基　　金：四川省科技厅资助项目(2010JY0080);四川省卫生厅资助项目(80173)

摘　　要：目的:采用抗体芯片技术检测在大鼠肾间质纤维化过程中炎症因子的变化情况,以筛选出可能的致病因子,并进一步研究其可能的致病机制。方法:将20只大鼠随机分为假手术组和单侧输尿管结扎组,分别于术后第7d、14d处死5只大鼠,取肾脏组织做抗体芯片检测并筛选出可能的致病因子。之后,对致病因子进行干预并观察疗效。结果:肾间质纤维化时MCP-1、Fractalkine和TIMP-1的变化最为显著。针对Fractalkine的干预效果明显。结论:①抗体芯片技术可以用于筛选肾间质纤维化的致病因子。②辛伐他汀能拮抗Fractalkine的特异性受体而延缓肾间质纤维化进程。Objective： To detect the overall changes of inflammatory factors in renal interstitial fibrosis of rats through antibody microarray technology,then to screen out potential pathogenic factors for further studying its pathogenic mechanism.Methods：20 rats were randomly divided into sham operation group and unilateral ureteral obstruction group.5 rats of each group were randomized to be killed on the 7th and 14th day after the operation.Their renal tissue was detected with the antibody microarray technology to find potential pathogenic factors.The next study was to intervene pathogenic factors and observe the curative effect.Results：The changes of MCP-1,Fractalkine and TIMP-1 were the most significant.And the intervention effect towards Fractalkine was significant too.Conclusions：①Antibody microarray technology can be used to define potential therapeutic target of disease.②Simvastatin may remit the progress of renal interstitial fibrosis by inhibiting Fractalkine’s specific receptor.

关 键 词：抗体芯片 炎症因子 RIF 单侧输尿管结扎 辛伐他汀 

分 类 号：R365[医药卫生—病理学] R692[医药卫生—基础医学]