在GDNF基因修饰的骨髓基质细胞与缺氧复氧神经细胞的共培养中神经细胞凋亡的变化  

EXPERIMENTAL RESEARCH ON CO-CULTIVATION OF BONE MARROW MESENCHYMAL STEM CELLS MODIFIED BY GDNF GENE AND NEURONS SUBJECTED TO ANOXIA-REOXYGENATION

作　　者：熊怀林[1] 杨朝鲜[2] 高小青[2] 邓莉[2] 

机构地区：[1]泸州医学院人体解剖学教研室,四川泸州646000 [2]泸州医学院神经生物学研究室,四川泸州646000

出　　处：《泸州医学院学报》2011年第5期552-555,共4页Journal of Luzhou Medical College

基　　金：四川省科技厅资助项目(2006J13-005-1);四川省教育厅资助项目(2006A155)

摘　　要：目的:探讨胶质源性神经营养因子(GDNF)基因修饰的骨髓基质细胞(BMSCs)对缺氧复氧神经细胞的保护作用。方法:GDNF基因修饰的BMSCs(BMSCs/GDNF)经诱导后与缺氧复氧神经细胞共培养,采用Hoechst33258检测神经细胞凋亡在共培养细胞中的表达。结果:BMSCs/GDNF组的神经细胞凋亡率显著低于BMSCs组和缺氧组(P<0.05)。结论:BMSCs/GDNF通过抑制缺氧复氧神经细胞凋亡发挥神经保护作用。Objective：To study the protective effects of BMSCs modified by GDNF gene（BMSCs/GDNF）to neurons subjected to anoxia-reoxygenation.Methods：BMSCs/ GDNF induced into neural-like cells were co-cultured with neurons subjected to anoxia-reoxygenation.Hoechst33258 staining was used to detect apoptosis of neurons in co-cultured cells.Results：The rate of apoptosis of neuron in BMSCs/GDNF group was lower than that in BMSCs group and anoxia group（P〈0.05）.Conclusion：BMSCs/GDNF have neuroprotective effects due to inhibiting apoptosis of neurons subjected to anoxia-reoxygenation.

关 键 词：GDNF BMSCS 缺氧复氧 神经细胞 共培养 

分 类 号：R741.05[医药卫生—神经病学与精神病学]