上皮型钙黏附蛋白、β-连接素在结直肠癌及转移灶组织中的表达及临床意义  

Expression of E-cadherin and β-catenin in primary colorectal carcinoma and metastatic lymph nodes and it＇s clinical significance

作　　者：陈健智[1] 李璇[1] 姚岐[1] 曹修淮[1] 

机构地区：[1]南方医科大学附属南海医院病理科,广东省佛山528200

出　　处：《中国基层医药》2011年第15期2022-2024,I0004,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省佛山市卫生局医学科研项目（2009209）

摘　　要：目的探讨上皮型钙黏附蛋白（E-cadherin）和β-连接素（β-catenin）在结直肠癌及转移灶组织中的表达及临床意义。方法用免疫组织化学方法检测E-cadherin和β-catenin在20例正常结肠黏膜（A组）、25例无淋巴结转移的结直肠癌（B组）、39例伴淋巴结转移的结直肠癌原发灶和同一个体肠系膜淋巴结转移灶组织（C组）中的表达情况，观察E—cadherin和β-catenin表达与肿瘤转移及预后的相关性。结果B组和C组E-cadherin表达阳性率均明显低于A组（U=4．681、U=5．775，均P=0．000）；C组原发灶中E．cadherin的表达强阳性率（5．1％）明显低于B组（16．O％）（U=2．149，P=0．032），C组转移灶与原发灶组织中E．cad-herin的表达差异无统计学意义（P=0．186）；β-eatenin在B组与C组、C组原发灶与转移灶组织中的表达差异均无统计学意义（P=0．235、P=0．715）；c组E—cadherin胞膜的表达下调和β-catenin胞膜的表达下调显示正相关（r=0．387，P=0．024），B组E—cadherin胞膜的表达下调和β-catenin胞膜的表达下调无明显相关性〈r=0．080，P=0．723）。结论E—Cadherin表达下调与结直肠癌伴有淋巴结转移有关，且与β-catenin的表达下调有明显的相关性，而β-catenin表达与结直肠癌伴淋巴结转移无关。Objective To investigate the expression of E-cadherin and β-catenin in the primary colorectal carcinoma and metastatic lymph nodes and it＇s clinical significance. Methods Immunohistochemical analysis of E- cadherin and β-catenin was performed in colorectal mucosa（ n =20,group A） ,colorectal carcinoma without metastasis （ n = 25, group B） and colorectal carcinoma with metastatic lymph nodes （n = 39, group C ） The expression of E-cadherin and β-eatenin and the metastasis and prognosis of colorectal carcinoma were observed. Results The strongly positive expression of E-cadherin in primaries of group C（5.1% ） was obviously lower than in group B（ U = 2. 149, P = 0.032）, whereas no statistic differences of the expression of E-cadherin between primaries and metastases of group C were observed（P =0. 186）. There was no difference of the expression of β-catenin between group B and group C, the same of primary colorectal carcinoma and self control lymph nodes of group C （P = 0.235 and P = 0. 715 ）. There was positive correlation between membranous down regulation of E-cadherin and β-catenin in group C （r = 0.387, P = 0.024）, but no relationship was found in group B （ r = 0. 080, P = 0.723）. Conclusion The decreased membranous expression of E-cadherin in the colorectal carcinoma was correlated with lymph nodes metastasis, and was obviously related to membranous down regulation of β-catenin. There was no relevance between the expression of β-catenin and the lymph nodes metastasis of colorectal carcinoma.

关 键 词：结直肠肿瘤 E-钙黏蛋白 Β-连接素 肿瘤转移 

分 类 号：R735[医药卫生—肿瘤]