107例多发性骨髓瘤M蛋白检测与免疫学分型分析  

M Protein Detection and Immunology Typing Analysis of 107 Patients with Multiple Myeloma

作　　者：刘玉梅[1] 赵有利[1] 石翀[1] 李光迪[1] 

机构地区：[1]兰州大学第二医院检验科,兰州730030

出　　处：《现代检验医学杂志》2011年第4期132-134,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨免疫固定电泳技术在鏊定M蛋白上的应用。方法采用血清蛋白电泳技术、血清免疫固定技术（IFE）检测107例MM患者血清M蛋白。结果107例MM患者中97例血清蛋白电泳检出M蛋白，107例免疫固定电泳均检出M蛋白。M蛋白IFE分型结果为IgG型63例，其中IgG κ型29例，IgGλ型34例；IgA型25例，其中IgA κ型11例、IgAλ型14例；IgM型3例，其中IgMκ型1例，IgMλ型2例;单纯轻链型15倒，其中K型2例，X型13例。60例MM患者中有39例检出尿本-周氏蛋白，其中，12例为κ型，27例为λ型。三种方法中血清免疫固定电泳的检出率最高，血清蛋白电泳次之，尿液本周氏蛋白电泳检出率最低（P〈O．01）。结论采用免疫固定电泳技术，探作简单、快速，灵敏度高，沉淀带客易识别，是鉴定各类型M蛋白的较准确、直观的有效方法。Objective To study the application of immunofixation electrophoresis （IFE） in typing M proteins. Methods Serum M proteins were detected in 107 patients By serum protein electrophoresis and IFE. Results Among I07 patients with MM,96 of them were affirmed to had M band with serum protein electrophoresis but M bands appeared in all of the MM patients with IFE. IFE manifest：there were IgG 63 cases （IgG κ 29 and IgG λ 34）,IgA 25 cases （IgA κ11 and IgAλ ）, 14） ,IgM 3 cases （IgM κ 1 and IgM λ, 2） and free light chain 15 cases （r 2 and }, 13）. Of 60 cases of MM,39 patients were detected to had Bence Jones proteins （12 κ and 27λ） in urine by IFE. The serum IFE had the highest sensitivity in identification M protein among the three methods. The second was serum protein electrophoresis and the lowest was the urine Bence Jones proteins （P ~ 0.01）. Conclusion The technology of IFE, characterized by easy differentiation of electrophoretic bands ,simple operation,high sensitivity and rapidity,has great value in typing M proteins.

关 键 词：多发性骨髓瘤 M蛋白 血清蛋白电泳 免疫固定电泳 本周氏蛋白 

分 类 号：R730.43[医药卫生—肿瘤]