Cloning of WRKY2 Gene in Tomato  

番茄WRKY2基因的克隆(英文)

作　　者：李娜[1] 于涌鲲[1] 赵福宽[1] 汤世坤[1] 孙清鹏[1] 于同泉[2] 路苹[2] 

机构地区：[1]北京农学院生物技术学院,北京102206 [2]农业应用新技术北京市重点实验室,北京102206

出　　处：《Agricultural Science & Technology》2011年第8期1125-1128,共4页农业科学与技术（英文版）

基　　金：Supported by Beijing Nature Science Foundation(5102015)~~

摘　　要：[Objective]The aim was to explore the function of WRKY transcription factor in tomato.[Method]The primers were designed in this study according to the obtained WRKY fragments,and the total RNA from tomato treated with 100 μmol/L of JA for 6 h was used as the template for RT-PCR.[Result]The 608 bp fragment was obtained from tomato with RT-PCR method.Sequence analysis indicated that this sequence contained WRKYGQK conservative domain and the similarity with Capsicum annuum WRKY-c and Nicotiana tabacum NtWRKY-7 were 79% and 74%,respectively.[Conclusion]WRKY gene sequence in tomato was cloned successfully.[目的]研究WRKY转录因子在番茄中的功能。[方法]根据已克隆的番茄WRKY转录因子片段设计引物,以100μmol/LJA处理6h后的番茄总RNA为模板,采用RT-PCR方法。[结果]从JA诱导的番茄幼苗中获得了608bp的cDNA片段,序列分析表明,该序列含有WRKYGQK保守结构域,与Capsicum annuum WRKY-c序列相似性是79%,与Nicotiana tabacum NtWRKY-7序列相似性是74%。[结论]已经成功克隆了番茄WRKY2基因片段。

关 键 词：TOMATO WRKY transcription factor WRKY2 gene CLONING 

分 类 号：S641.2[农业科学—蔬菜学]