壳寡糖诱导甘蔗叶多酚与防御酶活性的变化  

Chitosan oligosaccharide induced changes in polyphenols and defense enzymes activities in sugarcane leaves

作　　者：周桂[1] 靳晓芸[1] 邓光辉[1] 丘立杭[2,3] 邹成林[1] 李杨瑞[2,3,4,5] 

机构地区：[1]广西民族大学,南宁530007 [2]广西亚热带生物资源保护利用重点实验室 [3]广西大学农学院,南宁530005 [4]中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室 [5]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《南方农业学报》2011年第8期874-877,共4页Journal of Southern Agriculture

基　　金：国家科技支撑计划项目(2007BAD30B03);广西科技攻关重点项目(桂科攻0782004-3);广西科技攻关项目(桂科能0815011);广西自然科学基金项目(2010GXNSFA013101);广西教育厅项目(200707MS034);广西民族大学引进人才项目(2010QD022)

摘　　要：【目的】探讨壳寡糖对甘蔗抗性相关次生代谢物及相关防御酶的影响。【方法】以甘蔗品种新台糖22号(ROC22)为材料,拔节初期对甘蔗叶片分别喷施25、50和75mg/L壳寡糖溶液,以喷施清水为对照(CK)。喷施1、2、3、4d后分别取+1叶测定壳寡糖诱导甘蔗叶多酚与防御酶(苯丙氨酸解氨酶、多酚氧化酶和过氧化物酶)活性的变化。【结果】50～75mg/L壳寡糖处理有利于甘蔗叶多酚积累;50mg/L壳寡糖处理2d后,苯丙氨酸解氨酶活性最大,为对照的1.8倍;50mg/L壳寡糖处理4d后,多酚氧化酶活性最高,为对照的1.7倍;75mg/L壳寡糖处理有利于提高甘蔗叶片过氧化物酶活性。【结论】50～75mg/L壳寡糖可以诱导甘蔗叶总多酚的积累及提高相关防御酶活性,这有利于提高甘蔗的整体抗性。[Objective]The present study was carried out to explore the effect of chitosan oligosaccharide on secondary metabolites and some defense enzyme activities related to stress resistance in sugarcane. [Method]The leaves of sugarcane variety ROC22 were treated with 0 （CK）, 25, 50 and 75 mg/L chitosan oligosaccharide solution at the early elongation stage. The polyphenol content and the activities of defense enzymes, viz., phenylalanine ammonialyase （PAL）, polyphenol oxidase （PPO） and peroxidase （POD） were measured in ＋1 leaf of sugarcane at 1, 2, 3 and 4 d after spraying. [Result]50-75 mg/L chitosan oligosaccharide treatment was conducive to the accumulation of polyphenol in sugarcane leaf. The PAL activity reached to its maximum and was 1.78 times higher than the control after 2 d of 50 mg/L chitosan oligosaccharide treatment. The PPO activity reached to its maximum and was 1.7 times higher than the control after 4 d of 50 mg/L chitosan oligosaccharide treatment. The 75 mg/L chitosan oligosaccharide treatment was conducive to improve POD activity of sugarcane leaf. [Conclusion]It was found that 50-75 mg/L chitosan oligosaccharide treatment can improve the polyphenol accumulation and the activities of defense enzymes in sugarcane leaves, which is helpful for improving sugarcane resistance to various biotic and abiotic stresses.

关 键 词：甘蔗 壳寡糖 多酚 苯丙氨酸解氨酶 多酚氧化酶 过氧化物酶 

分 类 号：S566.101[农业科学—作物学]