正交设计法优化培养基中美拉德反应条件以提高猪苓菌核产量的研究  

Study on Optimization of Maillard Reaction Conditions in Culture Medium to Enhance Yield of Polyporus umbellatus Sclerotium by Orthogonal Design

作　　者：尹婉嫱[1] 郭顺星[1] 

机构地区：[1]中国医学科学院-中国协和医科大学药用植物研究所,北京1000193

出　　处：《中国药学杂志》2011年第17期1309-1311,共3页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金项目(30830117);国际科技合作项目(2011DFA31260)

摘　　要：目的用正交设计法优化培养基中美拉德反应的条件,以提高猪苓菌核产量。方法以果糖和精氨酸作为美拉德反应的原料,经过调节pH后在一定温度下独立灭菌一定时间,之后与含蛋白胨和无机盐的培养基混合均匀并用于诱导猪苓菌核。以菌核干重为指标,对灭菌前pH值,果糖与精氨酸浓度比,灭菌时间(min),灭菌温度(℃)4个因素进行了L9(34)正交实验。结果极差分析结合方差分析表明,灭菌前pH值对菌核产量具有显著影响。优化后反应条件为:灭菌前pH为10;浓度比为200∶1;灭菌时间为80 min;灭菌温度为121℃。菌核产率提高了75%,菌核干重提高到每皿(0.225±0.16)g。结论相比优化前菌核产率为30%～50%,菌核干重为每皿0.1～0.15 g,新的反应条件显著提高了菌核产量。OBJECTIVE To optimize Maillard reaction conditions in culture medium by orthogonal design so as to enhance yield of Polyporus umbellatus sclerotium. METHODS According to our previous study, it was found that Maillard reaction products in fructose medium might play a role in sclerotial induction in P. umbeUatus. Maillard reaction products, as indicated in the references, varied with reaction time, carbon-nitrogen ratio in reactants, as well as pH value and temperature in reaction system. In this study, Fructose and arginine of different concentration ratios were mixed and used as reactants for Maillard reaction after pH value adjustment, which were sterilized for some time under a specific temperature. The mixture was subsequently mixed with medium containing peptone and mineral salts. The ultimate medium was used for inducing sclerotium of P. umbellatus. Maillard reaction conditions were optimized by a L9 （34） orthogonal design, in which, pH value before sterilization （A）, concentration ratio（ B）, sterilization time （C）and sterilization temperature（D） were the four factors to modulate. Each factor had three levels. Sclerotium dryweight was the index for statistic analysis. RESULTS The results of range analysis and variance analysis showed that the pH value before sterilization（A） significantly affected sclerotium dryweight. The final optimized conditions were： pH of reaction system was 10 before sterilization;concentration ratio of fructose and arginine was 200：1 ;sterilization time was 80 min; and sterilization temperature was 121℃. The sclerotium yield was 75% higher than that before optimization 30% -50%, while the sclerotium dryweight increased from 0. 1 -0. 15 g per dish to （0. 225±0. 16） g per dish. CONCLUSION The optimized Maillard reaction conditions greatly enhance P. umbellatus sclerotium formation, which will be used in the future research.

关 键 词：猪苓 菌核 美拉德反应 正交设计法 

分 类 号：R931.2[医药卫生—生药学]