谷氨酸棒杆菌ATCC13032原生质体的制备与再生  

Protoplast preparation and regeneration of corynebacterium glutamicum ATCC13032

作　　者：张媛媛[1,2] 刘建忠[2] 

机构地区：[1]广东轻工职业技术学院食品系,广州510300 [2]中山大学生命科学学院,广州510300

出　　处：《中国调味品》2011年第9期41-44,50,共4页China Condiment

基　　金：国家自然科学基金项目(20876181)

摘　　要：以谷氨酸棒杆菌ATCC13032为出发菌,研究了青霉素浓度及溶菌酶浓度对原生质体形成率的影响,以及氯化钙和二甲基亚砜等物质的添加对其再生率的影响。结果表明最佳青霉素浓度及溶菌酶浓度分别为0.6U/mL和1mg/mL,在此条件下谷氨酸棒杆菌ATCC13032原生质体形成率达96%。为进一步提高原生质体再生率,尝试了4种添加物,其中氯化钙及二甲基亚砜能显著提高原生质体再生率。当3g/L氯化钙和10mL/L的二甲基亚砜组合添加时原生质体再生率更可达34%,是未添加的2.1倍,达到采用夹层法培养能获得再生率的最大值。The strain corynebacterium glutamicum ATCC 13032,a typical corynebacterium glutamicum strain,was used to prepare protoplast.Penicillin concentration,lysozyme concentration and additives were examined systematically.When penicillin concentration,lysozyme concentration were 0.6U/mL and 1 mg/mL,the rate of obtained protoplasts was 96%.For enhancing the regeneration frequency of the protoplast,4 kinds of additives were applied.Within the scope studied,both CaCl2 and DMSO were shown to be effective.The most preferable conditions of the additives were the combination of 3 g/L CaCl2 and 10 mL /L DMSO,the maximum regeneration frequency of the protoplast reached 34%,which was 2.1 folds than that in basal medium.

关 键 词：谷氨酸棒杆菌ATCC13032 原生质体 再生 

分 类 号：TS264.23[轻工技术与工程—发酵工程]