谷氨酰胺对内毒素血症大鼠心功能影响的研究  

Effect of Glutamine on myocardial function of endotoxemia rat

作　　者：黄日红[1] 刘鹏程[2] 曲鹏[2] 

机构地区：[1]大连医科大学附属第一医院心脏外科ICU,辽宁大连116011 [2]大连医科大学附属第二医院心内科,辽宁大连116011

出　　处：《中国现代医学杂志》2011年第23期2817-2820,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(No:30670836)

摘　　要：目的探讨谷氨酰胺(Gln)对脂多糖(LPS)致大鼠心功能障碍的保护作用。方法雄性健康清洁级大鼠70只,随机分为对照组(生理盐水5mL)10只、LPS组(10mg/kg)30只、Gln(LPS10mg/kg+Gln1g/kg)治疗组30只,腹腔注药后6、24和48h测定左室收缩末期压力(LVESP)、左室压力上升及下降最大变化速率(±dp/dtmax)等心功能指标;RT-PCR检测TNF-αmRNA表达;ELISA检测血清肌钙蛋白(IcTnI)、TNF-α、B型钠尿肽前体氨基端肽(NT-proBNP)含量。结果 LPS可使心肌TNF-αmRNA表达增高,同时导致血清cTnI、TNF-α、NT-proBNP含量升高及左室功能减退,Gln能下调LPS诱导的TNF-αmRNA表达,使cTnI、TNF-α、NT-proBNP降低并部分改善左室功能。结论 Gln对LPS所致心肌损伤及心功能减退有一定的保护作用。【Objective】 To explore the protective effect of Glutamine（Gln） on endotoxemia rat myocardial dysfunction.【Methods】 70 clean grade healthy male rats were randomly divided into three groups： 10 in control group（NS 5 mL）,30 in LPS group（10 mg/kg） and 30 in Gln treatment group（LPS 10 mg/kg,Gln 1 g/kg）.The functional indexes of heart,such as left ventricular end systolic pressure（LVESP） and the maximum velocity of intraventricular pressure rise and decline（±dp/dtmax） was examined respectively at 6,24 and 48 hrs.The expression of TNF-α mRNA was detected by RT-PCR.The levels of cTnI,TNF-α and NT-pro BNP were determined by ELISA.【Results】 LPS could increase the expression of TNF-α mRNA in rat myocardium and the levels of cTnI,TNF-α and NT-pro BNP in serum and induce the left ventricular dysfunction.Gln could down regulate the LPS induced expression of TNF-α mRNA,decrease the levels of cTnI,TNF-αand NT-pro BNP,improve the left ventricular function partly.【Conclusion】 Gln has a protective effect on LPS-induced myocardial dysfunction.

关 键 词：内毒素血症 谷氨酰胺 心功能 

分 类 号：R-332[医药卫生] R331.31