凝血酶转化人支气管上皮细胞形态学改变研究  

The features of cytological morphometry of thrombin transformed human papillomavirus-18 immortalized human bronchial epithelial cells

作　　者：曹守冬 肖伟[2] 

机构地区：[1]煤炭总医院急诊科,北京100028 [2]山东大学齐鲁医院呼吸科,山东济南250012

出　　处：《现代肿瘤医学》2011年第9期1731-1734,共4页Journal of Modern Oncology

摘　　要：目的:探讨凝血酶转化永生化人支气管上皮细胞(BEP2D细胞)形态学改变。方法:选择典型Giem-sa染色的细胞克隆图片,应用四川大学设计的MIAS-300型图像分析系统,随机选取视野内形态完整的细胞和细胞核进行测定。结果:发生恶性转化的细胞克隆细胞形态发生变化,与非转化型细胞克隆的细胞形态比较,细胞及细胞核面积、周长、最大直径、最小直径、等效直径及形状因子、异形指数、细胞核与细胞浆之比之间均有显著性差异。结论:凝血酶转化永生化人支气管上皮细胞具有恶性细胞表型。Objective：To investigate the features of cytological morphometry of thrombin transformed human papillomavirus-18 immortalized human bronchial epithelial cells（BEP2D cells）.Methods：Cell and nuclear morphometry were measured by a computer-assisted image analyser system on Giemsa-stained cytological specimens.The following cell and nuclear morphometric parameters were analysed： area,perimeter,maximum diameter and minimum diameter,equivalent diameter,form factor,form irregular index,and ratio of nucleus to plasma.Results：There were statistically differences in all of these parameters when thrombin-transfor-med BEP2D cells were compared with control.The transformed cell volume became obviously bigger and the nuclei shape became more and more rounded than control.Same changes were observed with NNK-transformed BEP2D cells.Conclusion：The results indicated that malignant cytological morphometry could be observed in thrombin-transformed BEP2D cells.

关 键 词：凝血酶 恶性转化 永生化人支气管上皮细胞 细胞形态学 

分 类 号：R730.3[医药卫生—肿瘤]