载多西紫杉醇脂质微泡联合超声靶向微泡破裂对兔VX2肝癌微血管的作用  

Effect of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on microvessel of rabbit VX2 liver tumor models

作　　者：康娟[1] 吴小翎[2] 张勇[3] 冉海涛[4] 王志刚[4] 

机构地区：[1]重庆医科大学附属第二医院肝病研究中心,重庆400010 [2]重庆医科大学附属第二医院消化内科,重庆400010 [3]重庆医科大学附属第一医院超声科,重庆400016 [4]重庆医科大学超声影像学研究所,重庆400010

出　　处：《中国介入影像与治疗学》2011年第5期431-434,共4页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家自然科学基金委员会科学部主任基金(81041064);国家科技重大专项课题(2008ZX10002-006)

摘　　要：目的探讨载多西紫杉醇脂质微泡(DLLM)联合超声靶向微泡破裂(UTMD)对兔VX2肝癌微血管的抑制作用。方法建立60只兔VX2肝癌动物模型,将其随机分为6组(n=10):单纯药物组(Doc组)、单纯载药微泡组(DLLM组)、药物+超声组(Doc+US组)、单纯微泡+超声组(PLM+US组)、载药微泡+超声组(DLLM+US组)及对照组,比较各组动物肿瘤组织的血管密度(MVD)、CD34及VEGF的表达。结果处理后DLLM+US组的CD34表达水平低于其他各组,MVD明显降低,与其他各组相比差异有统计学意义(P<0.01);DLLM+US组的VEGF表达水平明显低于其他各组(P<0.01)。结论 DLLM联合UTMD能抑制兔VX2肝癌的微血管生成,从而抑制兔VX2肝癌的生长。Objective To observe the inhibition effect of docetaxel-loaded lipid microbubbles（DLLM） combined with ultrasound targeted microbubbles destruction（UTMD） on microvessel in rabbit VX2 liver tumor models.Methods Sixty rabbits were randomly divided into 6 groups（n=10）,i.e.Doc group（used docetaxel only）,DLLM group（used docetaxel-loaded lipid microbubbles）,Doc＋US group（used docetaxel combined with ultrasound positioning irradiation）,PLM＋US group（used microbubbles combined with ultrasound positioning irradiation）,DLLM＋US group（used docetaxel-loaded lipid microbubbles combined with ultrasound positioning irradiation） and control group.The expression of CD34 and VEGF and microvessel density（MVD） were compared among different groups.Results After treatment,the expression of CD34 in DLLM＋US group was lower,the MVD of DLLM＋US group was markedly lower than that of the other groups（P0.01）,while the expression of VEGF in this group was the lowest among all 6 groups（P0.01）.Conclusion DLLM combined with UTMD can inhibit the generation of microvessels in rabbit VX2 liver tumor,thus inhibit the growth of the tumor.

关 键 词：多西紫杉醇 微泡 微血管 兔 肝肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]