黄连生地方防治放射性唾液腺损伤的实验研究  

Experimental study of Huanglianshengdi decoction on radioprotection of salivary glands

作　　者：傅辰春[1] 王晓萍[1] 华海清[2] 赵云艳[1] 张新良[1] 丁巍[1] 

机构地区：[1]解放军八一医院全军肿瘤中心放疗科,南京210002 [2]解放军八一医院全军肿瘤中心肿瘤内科,210002

出　　处：《临床肿瘤学杂志》2011年第8期689-692,共4页Chinese Clinical Oncology

基　　金：南京军区医学科技创新基金资助重点项目(2007年度)

摘　　要：目的观察中药复方制剂黄连生地方对放射性唾液腺损伤的防治作用。方法将28只新西兰大白兔随机分为4组:空白对照组、单纯照射组、中药+照射组和氨磷汀+照射组,每组7只。照射采用6MV X线双侧腮腺区15Gy单次照射。中药+照射组于照射前3天开始予中药灌胃(10m l/kg)至照射后3天,氨磷汀+照射组于照射前30m in由耳缘静脉注射氨磷汀(100mg/kg),空白对照组予等量生理盐水灌胃。全部实验动物于照射后第4天处死。HE染色后光镜下观察各组腮腺组织的病理学改变。应用放射性核素99TcmO4-显像方法检测各组处理前后腮腺摄取率、摄取比值和排泄率。结果光镜下观察显示,空白对照组腮腺腺泡细胞正常,单纯照射组的损伤程度较中药+照射组及氨磷汀+照射组严重,后两组损伤程度相似。腮腺功能研究表明,单纯照射组照射前后腮腺放射性核素99TcmO4-的摄取率分别为(88.37±21.15)%vs.(33.44±19.45)%,摄取比值分别为1.615±0.169vs.1.347±0.226,排泄率分别为(56.17±16.26)%vs.(38.15±23.82)%,差异均有统计学意义(P<0.05);中药+照射组处理后的摄取率、摄取比值及排泄率分别为(66.58±23.89)%、1.606±0.166和(44.56±17.35)%,氨磷汀+放射组分别为(72.87±27.61)%、1.495±0.106和(45.69±19.90)%,与单纯放射组处理后比较,差异均有统计学意义(P<0.05);中药+照射组和氨磷汀+放射组处理前后以及两组之间比较,差异均无统计学意义;空白对照组处理前后各项指标无明显变化。结论中药复方制剂黄连生地方具有减轻放射性唾液腺损伤作用,有望为临床防治提供一新的治疗方案。Objective To evaluate the protection of traditional Chinese medicine compound Huanglianshengdi decoction （ decoction） on radiation injury of salivary glands. Methods Twenty-eight rabbits were randomized into four groups： control group, irradiation group, radiation ＋ decoction group and irradiation ＋ amifostine group, with 7 rabits in each group. The X-ray radiation was per-formed with a dose rate of 3Gy/min, and a whole exposure of 15Gy was attained. The decoction（10ml/kg） was administrated 3 days before irradiation to 3 days afterward. Amifostine（100mg/kg） was administrated 30 min before irradiation via a marginal ear vein. Control group was treated by normal saline. Four days after radiation, all the rabbits were executed. The parotid gland was observed by light microscopy with HE-staining. The uptake rate, uptake index and ejection fraction of salivary glands were detected by active nueleus 99Tc^mO4^1. Results Microscopy showed that there was no injury of parotid acinar cells in control group. The injury in radiation group was worse than radiation ＋ decoction group and radiation ＋ amifostine group. And the injury in the latter two groups was similar. The function of salivary glands showed that the uptake rate before and after radiation was （88.37±21.15 ）% vs. （33.44 ± 19.45 ）% , the uptake index was 1. 615 ±0. 169 vs. 1. 347±0. 226 and the ejection fraction was （56. 17±16.26）% vs. （38. 15 ±23.82）%. There was a significant difference between them. The uptake rate, uptake index and ejection in radiation ＋ decoction group were （66. 58 ± 23.89） % , 1. 606 ± 0. 166 and（44. 56 ± 17.35 ） %, while in radiation ＋ amifostine group were （72. 87 ± 27.61 ） %, 1. 495 ±0. 106 and （45.69 ± 19. 90）%. There was no difference between before and after radiation in each group and between the two groups after radiation, but had difference compared with radiation group（P 〈 0. 05 ）. Control group showed no change before and after radiation. Conclusion The com

关 键 词：唾液腺 放射性损伤 黄连生地方 家兔 

分 类 号：R730.5[医药卫生—肿瘤]