Dynamic changes in cerebral microcirculation and hypoxia in the early stages of diffuse axonal injury  

作　　者：Jinning Song Xiaobin Liu Jingyu Chen Fenru Zhang Lei Xi 

机构地区：[1]Department of Neurosurgery, the First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China [2]Department of Nuclear Medicine, the First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China

出　　处：《Neural Regeneration Research》2011年第20期1530-1536,共7页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundationof China, No. 30471774;the Program for New Century Excellent Talents in University, Ministry of Education,China, No. NCET-05-0831

摘　　要：This study demonstrated that damage to the cerebral microvasculature, the formation of microthrombi and swelling of vascular endothelial cells occur early and peak 12 hours after injury in a rat model of diffuse axonal injury. Moreover, these pathological changes were most evident in the cerebral cortex. Cerebral microcirculatory dysfunction peaked later and had a shorter duration than axonal injury. In addition, the radioactive imaging agent, 99Tcm-4, 9-diaza-2, 3, 10, 10- tetramethyldodecan-2, 11 -dione dioxime, was used to visualize the dynamic changes that occur in tissue with cerebral hypoxia. The results demonstrated that cerebral hypoxia occurs at an early stage in diffuse axonal injury. Cerebral hypoxia was evident 12 hours after injury and declined slightly 24 hours after injury, but was significantly higher than in the control group. The pathological changes that underpin microcirculatory dysfunction did not occur at the same time as axonal injury, but did occur simultaneously with neuronal injury. Cerebral hypoxia plays a key role in promoting the secondary brain injury that occurs after diffuse axonal injury.This study demonstrated that damage to the cerebral microvasculature, the formation of microthrombi and swelling of vascular endothelial cells occur early and peak 12 hours after injury in a rat model of diffuse axonal injury. Moreover, these pathological changes were most evident in the cerebral cortex. Cerebral microcirculatory dysfunction peaked later and had a shorter duration than axonal injury. In addition, the radioactive imaging agent, 99Tcm-4, 9-diaza-2, 3, 10, 10- tetramethyldodecan-2, 11 -dione dioxime, was used to visualize the dynamic changes that occur in tissue with cerebral hypoxia. The results demonstrated that cerebral hypoxia occurs at an early stage in diffuse axonal injury. Cerebral hypoxia was evident 12 hours after injury and declined slightly 24 hours after injury, but was significantly higher than in the control group. The pathological changes that underpin microcirculatory dysfunction did not occur at the same time as axonal injury, but did occur simultaneously with neuronal injury. Cerebral hypoxia plays a key role in promoting the secondary brain injury that occurs after diffuse axonal injury.

关 键 词：diffuse axonal injury MICROCIRCULATION HYPOXIA 99Tcm-4  9-diaza-2  3  10  10- tetramethyldodecan-2  11-dione dioxime radioactive counting brain injury neural regeneration 

分 类 号：Q244[生物学—细胞生物学] Q426