大肠癌组织中VEGF-C、STAT3的表达变化及意义  

Expression and significance of VEGF-C,STAT3 mRNA and protein in colorectal cancer

作　　者：季兵[1] 戚峰[2] 邱宇杰[2] 赵娜[2] 刘彤[2] 

机构地区：[1]苏州大学附属第二医院,江苏苏州213001 [2]天津医科大学总医院

出　　处：《山东医药》2011年第34期22-24,共3页Shandong Medical Journal

基　　金：天津市自然科学基金资助项目(08JCYBJC08100)

摘　　要：目的观察大肠癌组织中血管内皮生长因子C(VEGF-C)、信号转导和转录激活因子3(STAT3)mRNA及其蛋白的表达变化,并探讨其临床意义。方法分别采用荧光实时定量PCR法和免疫组化法检测60例大肠癌组织及远癌切端大肠组织中的VEGF-C、STAT3 mRNA及其蛋白。结果大肠癌组织组织中VEGF-C、STAT3 mRNA表达量分别为0.81±0.331、.33±0.48,VEGF-C、STAT3蛋白阳性表达者分别为39例、42例;远癌切端大肠组织中分别为0.62±0.281、.04±0.30、15例4、例。两者比较,P均<0.05。大肠癌组织中,VEGF-C、STAT3 mRNA及其蛋白表达呈正相关(r分别为0.982、0.892,P均<0.01)。VEGF-C、STAT3 mRNA及其蛋白表达水平与大肠癌肿瘤分化程度、浸润深度、淋巴结转移及Dukes分期有关(P均<0.05)。结论大肠癌组织中STAT3、VEGF-C mRNA及其蛋白高表达。二者在大肠癌的淋巴结转移中发挥重要作用。Objective To investigate the mRNA transcription and protein expression of vascular endothelial growth factor C （ VEGF-C ） and signal transducers and activators of transcription 3 （ STAT3 ） in colorectal cancer, and study the clinical significance. Methods The levels of VEGF-C, STAT3 mRNA transcription and protein expression of carcinoma and surgical margin tissues gotten from 60 cases with CRC were determined by real-time PCR and immunohistochemical strain separately. Results The VEGF-C, STAT3 mRNA transcription values of tumor tissues were 0.81 ± 0.33, 1.33 ± 0.48, and VEGF-C, STAT3 protein positive expression were detected in 39 and 42 cases ; the values in surgical margin tissues were 0.62 ± 0. 28, 1.04 ± 0.30 and 15, 4 cases separately, P 〈 0.05. In colorectal cancer tissue ,the correlation of mRNA transcription and protein expression were positive （ values of r were 0. 982,0. 892 separately, all P 〈 0.01 ）. The levels of VEGF-C ,STAT3 mRNA transcription and protein expression were related with differentiation of colorectal cancer, the depth of tumor, lymphatic node metastasis as well as Dukes stage （ all P 〈 0.05 ）. Conclusions The STAT3, VEGF- C mRNA transcription and protein express highly in colorectal cancer tissues. STAT3, VEGF-C play an important role in lymph metastasis of colorectal cancer.

关 键 词：大肠肿瘤 大肠癌 血管内皮生长因子 信号转导和转录激活因子 

分 类 号：R735.3[医药卫生—肿瘤]