小脑顶核电刺激对心肌梗死大鼠脑组织5-羟色胺含量的影响  

Effects of fastigial nucleus electrical stimulation on serotonin contents in the brain tissues of rats after myocardial infarction

作　　者：张润峰[1] 李霞[2] 胡大一[3] 高文根[3] 魏毅东[4] 邢燕[4] 

机构地区：[1]四川省绵阳市第三人民医院心血管内科,绵阳621000 [2]复旦大学附属华山医院神经内科 [3]北京大学人民医院心脏中心 [4]同济大学心肺血管中心

出　　处：《山西医科大学学报》2011年第8期613-616,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(30571780);中国博士后基金资助项目(20100471798);绵阳市科技局资助科研项目(10S1-1)

摘　　要：目的通过研究小脑顶核电刺激(FNS)对心肌梗死(MI)大鼠脑组织神经递质5-羟色胺(5-HT)的影响,探讨5-HT在FNS干预心脏性猝死(SCD)中的作用。方法 SD大鼠90只,随机分为3组,每组30只:MI组,仅结扎左冠状动脉前降支(LAD);FNS组,预先FNS 1 h再予以LAD结扎;小脑顶核毁损组,毁损小脑顶核5 d后FNS 1 h,再行LAD结扎;各组又分MI后1,7,21 d三个亚组。另取8只设为假手术组。LAD结扎1,7,21 d后,应用荧光分光光度法测定大鼠脑组织内5-羟色胺含量。结果①LAD结扎1,7 d后,与假手术组比较,MI组和小脑顶核毁损组大鼠脑组织5-HT均明显降低(P<0.05);与MI组比较,FNS组大鼠脑组织5-HT明显增加(P<0.05);小脑顶核毁损组与MI组脑组织5-HT含量比较差异无统计学意义(P>0.05)。②LAD结扎21 d后,各实验组间大鼠脑组织5-HT含量比较无统计学差异。结论 FNS可增加心肌梗死大鼠早期脑组织5-HT含量,并可能通过增加5-HT含量来控制心脏性猝死的发生。Objective To investigate the effects of fastigial nucleus electrical stimulation（FNS）on the serotonin（5-hydroxytryptamine,5-HT） contents in brain tissues of rats after myocardial infarction（MI）,and to explore the role of 5-HT in the prevention of sudden cardiac death（SCD） after FNS.Methods Ninety SD rats were randomized into 3 groups with 30 rats in each group：MI group,FNS group and fastigial nucleus lesion group.In MI group,left anterior descending artery of rats was ligated.In FNS group,rats were pretreated with fastigial nucleus electro-stimulation for 1 h,and then left anterior descending artery was ligated.In fastigial nucleus lesion group,rats were treated with fastigial nucleus electro-stimulation for 1 h after fastigial nucleus was damaged for 5 d,and then left anterior descending artery was ligated.Each group was subdivided into 3 subgroups according to the three time points of 1,7,21 d after ligation of left anterior descending artery.Eight SD rats were chosen as the sham operation group.At days 1,7,21 after ligation of left anterior descending artery,the contents of 5-HT were determined in brain tissues with fluorospectrophotometry.Results ①The levels of 5-HT in MI group and fastigial nucleus lesion group were significantly lower than that in sham operation group at days 1,7 after myocardial infarction（P0.05）,but 5-HT in FNS group was significantly higher than in MI group（P0.05）.There was no significant difference in 5-HT between MI group and fastigial nucleus lesion group（P0.05）.② At day 21 after myocardial infarction,there was no significant difference in 5-HT levels among 4 groups.Conclusion FNS can increase the levels of 5-HT in brain tissues at an early stage after myocardial infarction,which may be one of mechanism in the prevention of SCD.

关 键 词：小脑顶核 电刺激 心肌梗死 5-羟色胺 心脏性猝死 

分 类 号：R363[医药卫生—病理学]