二烯丙基硫醚对大鼠局灶性脑缺血再灌注损伤的保护作用和治疗时间窗  

Protective effect of diallyl sulfide on cerebral ischemia/reperfusion injury in rats and therapeutic window of opportunity

作　　者：林雪梅[1] 赵涌[1] 赵敬[1] 陈晏林[1] 李孄[1] 喻姗姗[1] 巫静娴[1] 

机构地区：[1]重庆医科大学分子医学与肿瘤研究中心,重庆400016

出　　处：《重庆医科大学学报》2011年第8期904-906,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30900457;81070917)

摘　　要：目的:研究二烯丙基硫醚(Diallyl sulfide,DAS)对大鼠局灶性脑缺血再灌注损伤的保护作用和治疗时间窗。方法:DAS的保护作用研究,采用线栓法制备大鼠大脑中动脉阻断(Middle cerebral artery occlusion,MCAO)局灶性脑缺血模型,分别在造模前7 d采用腹腔注射法连续给予DAS 25、50、100、200 mg/kg,制备MCAO模型,缺血2 h,再灌注24 h,评价大鼠神经功能状态、脑水肿、脑梗死面积;DAS的治疗时间窗研究,采用MCAO局灶性脑缺血再灌注模型,分别在缺血1 h、再灌注损伤后2、4、6h腹腔注射DAS 50 mg/kg,缺血2 h,再灌注24 h,评价神经功能状态、脑水肿和脑梗死面积模型。结果:DAS 50 mg/kg,能最大程度的改善大鼠脑缺血再灌注后神经功能评分,减轻脑水肿和减少脑梗死面积;缺血后1 h开始用药,神经功能缺损评分明显减少,脑水肿及脑梗死面积也明显下降,再灌注2、4 h后给药,疗效下降,6 h给药,则无明显作用。结论:DAS对大鼠局灶性脑缺血再灌注损伤有保护作用,有效时间窗在缺血后1 h到再灌注损伤后2 h内。Objective：To investigate the protective effect of diallyl sulfide and the therapeutic window of opportunity for diallyl sulfide following focal cerebral ischemia/reperfusion injury in rats.Methods：For the protective effect of diallyl sulfide,focal cerebral ischemia-/reperfusion models in rats were induced by the transient occlusion of the middle cerebral artery（MCAO）for 2 h,followed by reperfusion for 24 h,and the animals were administered DAS25,50,100,200 mg/kg,ip,q d × 7 d before transient MCAO.The neurological deficit score,infarct size and brain edema were examined.For the therapeutic window of opportunity,the MCAO model was induced by transient occlusion for 2 h and reperfusion for 24 h.The drugs were administered at 1h after the onset of ischemia and 2,4,6 h after reperfusion respectively.The neurological deficit score,infarct size and brain edema were examined.Results：DAS 50 mg/kg,ip,q d × 7 d before MCAO significantly reduced the neurological deficit score,infarct size and brain edema.The administration of DAS beginning at 1 h after the onset of ischemia significantly reduced neurological deficit sore,infarct size and brain ischemia;starting at 2,4 h after reperfusion,the neuroprotective effects decreased,and starting at 6 h after reperfusion did not result in significant reduction of the above indexes for brain tissue.Conclusion：Diallyl sulfide has protective effect on cerebral ischemia/reperfusion injury.The therapeutic window of opportunity of diallyl sulfide in transient focal cerebral ischemia injury is between 1 h after the onset of ischemia and 2 h after reperfusion.

关 键 词：二烯丙基硫醚 脑缺血再灌注损伤 神经保护 时间窗 

分 类 号：R743.3[医药卫生—神经病学与精神病学]