稳恒磁场对体外培养的大鼠脑神经细胞影响的研究  

Experiment on influence of static magnetic field on rat cortical neurons

作　　者：马波[1] 陈丽娜[1] 何月涵[1] 吕亚娜[1] 

机构地区：[1]哈尔滨医科大学生物物理学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2011年第4期301-303,共3页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金资助项目(D2007-48)

摘　　要：目的观察稳恒磁场对体外培养的大鼠幼鼠脑神经细胞的影响。方法原代培养新生Wistar大鼠的脑皮质神经细胞,测定未照射对照组及30 mT和50 mT强度的稳恒磁场照射组细胞培养液中乳酸脱氢酶(LDH)含量;细胞内超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和NO含量。结果 30mT强度照射组和50 mT强度照射组与对照组细胞内SOD活性、MDA含量、NO含量均有显著性差异;细胞培养液上清中LDH含量有显著差异。结论一定强度的稳恒磁场对大鼠幼鼠脑神经细胞造成损伤,且损伤程度存在强度-效应关系。Objective To observe the effect of static magnetic field（SMF） on in vitro cultured cortical neurons of rat. Methods Cortical neurons of newborn Wistar rat were primary cultured. The levels of LDH in cell culture medium were examined in unradiated group and static magnetic field of 30 mT and 50 mT radiation group. The concentrations of malondialdehyde （MDA） were determined by thiobarbiturif acid reactive substance method. The activity of superoxide dismutase （SOD） and the content of NO were determined. Results The activity of SOD, the contents of MDA and NO in cells of 30 mT and 50 mT radiated group were significantly different than that of control group, and the level of LDH in culture medium radiated group was significantly different also. Conclusion Certain strength of static magnetic field has inhibitory effect on rat cortical neurons, and there is dose-effect relationship intension.

关 键 词：稳恒磁场 神经细胞 乳酸脱氢酶 超氧化物歧化酶 一氧化氮 

分 类 号：R363.12[医药卫生—病理学]