Slit2/Robo1信号对鸡胚早期神经管及体节发育的影响  

Role of Slit2/Robo1 signaling in development of neural tube and somites in early chick embryos

作　　者：王广[1,2] 王晓钰[1,2] 李艳[1,2] 王丽京[3] 雷健[1,2] 张笑坛[1,2] 耿建国[3] 杨雪松[1,2] 

机构地区：[1]暨南大学香港大学脑功能与健康联合实验室 [2]暨南大学医学院组织与胚胎学系,广东广州510632 [3]广东药学院血管生物学实验室,广东广州510224

出　　处：《中国病理生理杂志》2011年第8期1592-1596,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展计划(973)项目(No.2010CB529702);国家自然科学基金资助项目(No.30971493No.31071054No.30871304);中央高校基本科研业务费专项资金资助项目(No.21610601)

摘　　要：目的:探讨Slit2/Robo1对鸡胚早期神经管和体节发育的影响。方法:显微注射法将质粒注射入HH10期胚胎神经管内,活体胚胎细胞电穿孔方法转染胚胎半侧神经管,以另一侧神经管为对照侧,原位杂交及免疫荧光方法观察转染10 h后神经管的发育和神经嵴细胞迁移至体节的情况。结果:下调Robo1侧神经管发育较正常对照侧异常,同时发现Slug表达和神经嵴细胞迁移至体节路线发生改变。结论:Slit2/Robo1信号可能通过影响Slug基因表达,对胚胎早期神经管闭合、神经嵴细胞正常产生及迁移方向以及体节分化有重要作用。AIM ： To investigate the effects of Slit2/Robo1 signaling on the development of neural tube and somites in early chick embryos. METHODS ： Plasmid DNA was injected into the lumen of the neural tube from dorsal side of HH10 chick embryo using microinjection, and then in ovo electroporation was performed at half-side of neural tube while another side served as control. Subsequent 10 - hour incubation was carried on after transfection until the development of neural tube and neural crest cells migrating to somites were investigated using the methods of immunofluorescence and in situ hybridization. RESULTS： Blocking Slit2/Robo1 signaling resulted in abnormal development of neural tube, while the expression of Slug and neural crest cells migrating to somites pathway were abnormal as well. CONCLUSION ： Slit2/Robo1 signaling can affect the expression of Slug and play an important role in the fusion of neural fold, the trajectory of generation and migration of neural crest cells, and the differentiation of somites in early chick embryos.

关 键 词：Slit2/Robo1信号 神经管 神经嵴细胞 体节 生皮肌节 

分 类 号：R363[医药卫生—病理学]