高游离脂肪酸通过FOXO1途径抑制内皮祖细胞增殖并促进其凋亡  

Free fatty acid inhibits proliferation and promotes apoptosis of endothelial progenitor cells via FOXO1 pathway

作　　者：陈莉[1] 王红祥[1] 赵湜[1] 李娜[1] 张文静[1] 丁胜[1] 

机构地区：[1]武汉市中心医院内分泌科,湖北武汉430014

出　　处：《中国病理生理杂志》2011年第8期1615-1618,共4页Chinese Journal of Pathophysiology

基　　金：湖北省卫生厅科研资助项目(No.JX3A24)

摘　　要：目的:研究高游离脂肪酸(FFA)对糖尿病患者内皮祖细胞(EPCs)增殖及凋亡的影响,评价FFA在糖尿病血管并发症中的可能作用。方法:分离培养2型糖尿病患者EPCs(DM组)和正常对照组EPCs(对照组),加入不同浓度(0、10和50 mmol/L)的FFA。MTT法检测EPCs增殖,Annexin-V/PI法检测其凋亡。RT-PCR法和Western blotting法检测核转录因子FOXO1的表达水平。结果:随着FFA浓度的增加,DM组和对照组EPCs增殖率明显下降,而凋亡率明显增加,差异均显著(P<0.05)。相同FFA浓度下,DM组增殖率低于对照组(P<0.05)。随着FFA浓度的增加,DM组和对照组FOXO1的mRNA表达逐渐降低,差异显著(P<0.05)。结论:高FFA可能通过FOXO1途径抑制EPCs增殖并诱导其凋亡,可能在糖尿病血管并发症的发生中发挥作用。AIM： To investigate the effect of free fatty acid （FFA） on endothelial progenitor cells （ EPCs）, and to elucidate the role of FFA in the pathogenesis of diabetic peripheral vascular disease. METHODS： EPCs were isolated from the patients with type 2 diabetes mellitus （DM group） and normal healthy persons （control group）. Various concentrations of FFA were added to the culturing system. MTT assay was used to detect the proliferative rate. Apoptotic rate of EPCs was measured by Annexin V/PI assay. The expression of FOXO1 was determined by RT - PCR and Western blotting. RESULTS ： In a dose - dependent manner, FFA attenuated the proliferative activity of EPCs in both control group and DM group, while increased the apoptotic rates （P 〈 0. 05）. The expression of FOXO1 decreased under the condition of FFA exposure and the difference was significant （P 〈 0. 05 ）. CONCLUSION： FFA may be a new factor in the pathogenesis of diabetic peripheral vascular disease through FOXO1 pathway.

关 键 词：游离脂肪酸 内皮祖细胞 细胞增殖 细胞凋亡 叉头框O1蛋白 

分 类 号：R363[医药卫生—病理学]