电凝法制作大鼠脑卒中模型的改良研究  

Improved electrocoagulation method for establishing rat cerebral apoplexy model

作　　者：刘海朋[1] 胡永波[1] 张小东[2] 王娜[3] 谢鹏[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016 [2]川北医学院附属医院神经内科,南充637000 [3]华中科技大学同济医学院附属协和医院神经内科,武汉430022

出　　处：《第三军医大学学报》2011年第17期1798-1802,共5页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(973计划;2009CB918300)~~

摘　　要：目的对电凝制作大鼠脑卒中模型方法进行改良及评价。方法将26只成年雄性SD大鼠(体质量200～300 g)分成5组:Tamura手术组、Tamura假手术组、改良手术一组、改良手术二组、改良假手术组。分别使用Tamura法和改良电凝法制作脑卒中模型。术后对大鼠神经功能缺损,脑梗死面积和部位进行评价。结果与Tamura法比较,改良电凝法手术时间更短,术后大鼠没有出现颞下颌关节紊乱;改良手术一组大鼠没有出现明显的神经功能缺损,改良手术二组大鼠肢体瘫痪比较明显;改良手术一组大鼠脑梗死面积比较小,梗死范围局限于大脑皮层,改良手术二组梗死面积较大,梗死部位在基底节测区和大脑皮层。结论①改良电凝法更加简便快速,对设备和操作技术要求更低,对大鼠其他损伤更小。②使用改良电凝法阻断大脑中动脉的不同部位,可以实现不同面积和部位的脑梗死、满足不同的实验条件和目的。Objective To improve the electrocoagulation method for establishing a rat cerebral apoplexy model,and to evaluate the improved electrocoagulation method.Methods Rat cerebral apoplexy models were established with Tamura method and an improved electrocoagulation method separately.Twenty-six adult male Sprague-Dawley（SD） rats（body weights 200 to 300 g） were divided into five groups： a Tamura operation group,a Tamura sham-operation group（no middle cerebral artery occlusion）,a first improved operation group（middle cerebral artery occlusion in position 1）,a second improved operation group（middle cerebral artery occlusion in position 2）,and an improved sham-operation group（no middle cerebral artery occlusion）.After operation,the neurological deficit and cerebral infarction area and site of rats were tested.Results Compared with Tamura method,the improved electrocoagulation method had shorter operation time and did not induce temporomandibular joint disorder.No obvious neurological deficit was found in the rats of the first improved operation group,but obvious paralysis of limbs was found in the rats of the second improved operation group.The rats of the first improved operation group that had infarction confined in the cerebral cortex exhibited a smaller infarction area than the rats of the second improved operation group that had infarction in the cerebral cortex and lateral caudoputamen.Conclusion The improved electrocoagulation method is simpler and more efficient,has lower requirements for equipments and operation techniques,and causes less injury to rats.With middle cerebral artery occlusion at different sites,the improved electrocoagulation method can induce cerebral infarction in different areas and at different sites,and thus,is suitable for experiments with different conditions and purposes.

关 键 词：电凝法 动物模型 大脑中动脉闭塞 脑梗死 大鼠 

分 类 号：R-332[医药卫生] R363.124