体外负压培养对人BMSCs BMP-2、VEGF mRNA表达水平的影响  

Effects of negative pressure on the mRNA expression of BMP-2 and VEGF in human BMSCs cultured in vitro

作　　者：杨治[1] 朱养均 何鹏[1] 张银刚[2] 

机构地区：[1]陕西中医学院附属西电医院骨科,西安710077 [2]西安交通大学医学院第一附属医院骨科,西安710061

出　　处：《中国矫形外科杂志》2011年第17期1476-1479,共4页Orthopedic Journal of China

基　　金：国家自然基金资助项目(编号:30973063);中国博士后科学基金资助项目(编号:20070421123);陕西省卫生厅科研基金(编号:2010323);西安市卫生局科技立项(编号:J2010B31)

摘　　要：[目的]探讨体外负压培养对人骨髓间充质干细胞(bone marrow-derived stroma cells,BMSCs)骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)、血管内皮生长因子(vascular endothelial growth factor,VEGF)mRNA表达水平的影响。[方法]取第3代BMSCs分为实验组和对照组,实验组进行间歇性负压培养,设置压力为17 kPa,30 min/次,4次/d,干预2周;对照组于普通CO2培养箱中常规培养。免疫组织化学染色检测BMP-2、VEGF的表达水平,RT-PCR检测2周后以及终止负压后1、2、3 d BMP-2、VEGF mRNA表达水平。[结果]诱导2周后,BMSCs呈现出显著的成骨细胞特性,与对照组比较,实验组细胞BMP-2、VEGF mRNA表达水平显著提高,且差异均有统计学意义(P<0.05),负压终止后3 d,两组细胞BMP-2 mRNA表达水平无明显差异(P>0.05),VEGF mRNA却在负压培养终止后的3 d内与对照组比较仍有显著差异(P<0.05)。[结论]体外负压培养可以提高BMSCs BMP-2、VEGF mRNA的表达水平。[ Objective] To investigate the effects of negative pressure on the mRNA expression of bone morphogenetic protein -2 （BMP- 2） and vascular endothelial growth factor （VEGF） in human bone marrow- derived stroma cells （BMSCs） in vitro. [ Method ] The third passage cells were divided into negative pressure treatment group and control group. The treatment group was induced by negative pressure intermittently （pressure： 17 kPa,30 min/time, and four times a day）for two weeks. The control group was cultured in conventional condition. Assessment of BMP -2 and VEGF expression were done by immunohisto- chemical staining. The mRNA expressions of BMP - 2 and VEGF in BMSCs were analyzed by real - time polymerase chain reac- tion （PCR）. [ Result] BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pres- sure. In the treatment group,the mRNA expressions of BMP- 2 and VEGF were significantly increased（ P 〈 0. 05 ） after 2 weeks, compared with the control group. However,Three days after the exposure to 2 -week negative pressure, these was no longer signif- icant difference between the two groups （P 〉 0.05 ）, these was significantly difference on VEGF （P 〈 0. 05 ）. [ Conclusion ] Neg- ative pressure is capable of promoting the mRNA expression of BMP -2 and VEGF in human BMSCs in vitro.

关 键 词：负压 BMSCS BMP-2 VEGF 

分 类 号：R363[医药卫生—病理学]