Assessment of 188Re marked anti MHC class II antibody by peripheral blood mononuclear cells stimulated by donor alloantigen  

作　　者：DING Guo-ping CAO Li-ping LIU Jie LIU Da-ren QUE Ri-sheng ZHU Lin-hua ZHOU Yi-ming MAO Ke-jie HU Jun-an 

机构地区：[1]Department of Surgery, Second Affiliated Hospital, ZhejiangUniversity School of Medicine, Hangzhou, Zhejiang 310009,China

出　　处：《Chinese Medical Journal》2011年第16期2512-2516,共5页中华医学杂志（英文版）

基　　金：This research was supported by a grant from the National Natural Science Foundation of China （No. 30872501）.

摘　　要：Background Previous studies showed that anti MHC-II monoclone antibody （MAb） only had partial inhibiting effect of alloreactive mixed lymphocyte reaction （MLR） in vitro and it was unsteady and non-persistent. The aim of this research was to determine whether radioactive isotope 188Re marked MHC-II antibody could benefit the allograft acceptance in transplantation as compared to normal MHC-II antibody. Methods 188Re was incorporated to 2E9/13F（ab＇）2 which is against swine MHC class II antigen （MAb-~88Re）. Porcine peripheral blood mononuclear （PBMC） cells were examined for proliferation and cytokine mRNA expression after stimulation with MHC-II MAb or MAb-188Re. Results The proliferative response of recipient PBMCs in mixed lymphocyte reaction （MLR） to donor alloantigen showed that the stimulation index of MAb-188Re group was significantly lower than the MHC-II MAb group and control （P 〈0.05）. mRNA expression of interleukin 2, interferon y and tumor necrosis factor a （type 1 cytokines） was lower in MAb-188Re group than the MHC-II MAb group, while interleukin 10 （type 2 cytokines） was higher in MAb-~aSRe group in the first 24 hours. Conclusion MAb-188Re could help the graft acceptance by inhibiting T cell proliferation, lowering the expression of type 1 cytokines and elevating the type 2 cytokines produced by PBMC.Background Previous studies showed that anti MHC-II monoclone antibody （MAb） only had partial inhibiting effect of alloreactive mixed lymphocyte reaction （MLR） in vitro and it was unsteady and non-persistent. The aim of this research was to determine whether radioactive isotope 188Re marked MHC-II antibody could benefit the allograft acceptance in transplantation as compared to normal MHC-II antibody. Methods 188Re was incorporated to 2E9/13F（ab＇）2 which is against swine MHC class II antigen （MAb-~88Re）. Porcine peripheral blood mononuclear （PBMC） cells were examined for proliferation and cytokine mRNA expression after stimulation with MHC-II MAb or MAb-188Re. Results The proliferative response of recipient PBMCs in mixed lymphocyte reaction （MLR） to donor alloantigen showed that the stimulation index of MAb-188Re group was significantly lower than the MHC-II MAb group and control （P 〈0.05）. mRNA expression of interleukin 2, interferon y and tumor necrosis factor a （type 1 cytokines） was lower in MAb-188Re group than the MHC-II MAb group, while interleukin 10 （type 2 cytokines） was higher in MAb-~aSRe group in the first 24 hours. Conclusion MAb-188Re could help the graft acceptance by inhibiting T cell proliferation, lowering the expression of type 1 cytokines and elevating the type 2 cytokines produced by PBMC.

关 键 词：MHC class H antibody dendritic cells CYTOKINE 188Re  acute rejection 

分 类 号：S852.4[农业科学—基础兽医学] S852.43[农业科学—兽医学]