Identification and functional annotation of novel microRNAs in the proximal sciatic nerve after sciatic nerve transection  

作　　者：LI ShiYing YU Bin WANG YongJun YAO DengBing ZHANG ZhanHu GU XiaoSong 

机构地区：[1]Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 210063, China

出　　处：《Science China(Life Sciences)》2011年第9期806-812,共7页中国科学（生命科学英文版）

基　　金：supported by the National High Technology Research and Development Program of China (Grant No. 2006AA02A128);the National Natural Science Foundation of China (Grant No. 30870811);the Jiangsu Provincial Natural Science Foundation (Grant No. BK2008010);a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)

摘　　要：The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules that regulate gene expression at the level of translation. In this paper, we focus on the identification and functional annotation of novel microRNAs in the proximal sciatic nerve after rat sciatic nerve transection. Using Solexa sequencing, computational analysis, and quantitative reverse transcription PCR verification, we identified 98 novel microRNAs expressed on days 0, 1, 4, 7, and 14 after nerve transection. Furthermore, we predicted the target genes of these microRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the known time-frame of peripheral nerve injury and repair. Our data provide an important resource for further study of the role and regulation of microRNAs in peripheral nerve injury and regeneration.The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules that regulate gene expression at the level of translation. In this paper, we focus on the identification and functional annotation of novel microRNAs in the proximal sciatic nerve after rat sciatic nerve transection. Using Solexa sequencing, computational analysis, and quantitative reverse transcription PCR verification, we identified 98 novel microRNAs expressed on days 0, 1, 4, 7, and 14 after nerve transection. Furthermore, we predicted the target genes of these microRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the known time-frame of peripheral nerve injury and repair. Our data provide an important resource for further study of the role and regulation of microRNAs in peripheral nerve injury and regeneration.

关 键 词：MICRORNA proximal sciatic nerve RAT Solexa 

分 类 号：Q52[生物学—生物化学] Q421