Snail基因与食管癌细胞侵袭迁移能力的相关性分析  

The relativity between Snail gene and invasion and migration ability of esophageal carcinoma cell

作　　者：鲁秦安[1] 康龙丽[1] 

机构地区：[1]西安文理学院,陕西西安710065

出　　处：《中国医药导报》2011年第26期24-26,共3页China Medical Herald

摘　　要：目的:探索Snail基因与食管癌细胞侵袭迁移能力的关系。方法:体外合成Snail序列特异性双链小干扰RNA,转染人食管癌细胞EC9706,应用RT-PCR及Western blotting方法检测干扰效果,细胞划痕实验检测细胞迁移能力变化,Transwell小室侵袭实验检测细胞侵袭能力变化。结果:经Snail特异性siRNA干扰后细胞Snail基因及蛋白表达水平明显降低,细胞的侵袭迁移能力随之降低,差异均有统计学意义(均P<0.05)。结论:Snail基因与食管癌细胞的侵袭迁移能力有关,采用siRNA干扰Snail基因的表达能有效逆转食管癌细胞的侵袭迁移能力。Objective： To study the relativity between Snail gene and invasion and migration ability of esophageal carcinoma cells. Methods： The chemically synthetic siRNA duplexes targetting Snail was transiently transfected into EC9706 cells. After siRNA interfering cells were harvested, the expression of Snail was investigated by of RT-PCR and Western blotting. Migration rate was measured by wound healing assay. Furthermore, the ability of cell invasion was investigated using Transwell chambers assay. Results： Snail-siRNA effectively inhibited the expression of Snail in gene and protein level and the ability of cell invasion and migration decreased either, the difference was statistically significant （all P〈0.05）. Conclusion： Snail contribute to the invasiveness of esophageal carcinoma cell, siRNA interference the expression of Snail gene can reverse invasion and migration ability of esophageal carcinoma cells effectively.

关 键 词：食管肿瘤 SNAIL基因 RNA干扰 侵袭转移 

分 类 号：R735.1[医药卫生—肿瘤]