油菜种子含油量相关的脂肪酶基因BnSDP1克隆与表达分析  

Cloning and expression analysis of an oil-content related lipase(BnSDP1) gene in Brassica napus

作　　者：魏明玉[1] 周佳[1] 闫素珍[1] 王政[1] 谭小力[1] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013

出　　处：《中国油料作物学报》2011年第4期338-343,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家973项目(2006CB101600)

摘　　要：通过电子延伸和RACE(cDNA末端快速扩增)克隆得到一个与油菜种子含油量相关的脂肪酶基因,命名为BnSDP1,其开放阅读框为2 472bp,编码分子量为92kD的蛋白,预测等电点为6.75,具有保守的patatin结构域,跨膜域位于N端和中间,序列分析显示BnSDP1拥有GXSXG脂肪酶的基本特征序列GSSVG。利用RT-PCR技术分析发现,在种子发育的成熟期(即脱水期),BnSDP1在低油品系种子中的转录水平高于高油品系,在根、茎、叶、花中均有表达,但在根和叶片中表达量高,在根中尤其显著,且受蔗糖诱导上调表达。Oil-content related lipase gene in Brassica napus（designated as BnSDP1） was cloned by in-silico and RACE（rapid-amplification of cDNA ends）.The object was to understand the lipid degradation during late stage of seed development.The open reading frame（ORF） of BnSDP1 was 2 472bp long,encoding a 92kD protein,theoretical pI6.75.A transmembrane region located near N-terminal and middle region.The cloned BnSDP1 encoded a patatin domain and GXSXG lipase motif.Real time quantitative PCR（RT-PCR）showed expression patterns of BnSDP1 with high expression in low oil-content seeds at seed maturing stage.It expressed in stem,leaf and flower,and especially well in roots.Its transcript level was up-regulated by sugar induction in roots.

关 键 词：甘蓝型油菜 含油量 脂肪酶基因 序列分析 实时定量PCR 

分 类 号：Q781[生物学—分子生物学] S565.4[农业科学—作物学]