巨大芽孢杆菌A6对油菜根系分泌物所含有机酸和糖类的趋化性  

Chemotaxis of Bacillus megaterium strain A6 towards organic acid and saccharide from roots exudates of rapeseed

作　　者：胡小加[1] 谢立华[1] 余常兵[1] 李银水[1] 刘胜毅[1] 张春雷[1] 廖星[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学重点开放实验室,湖北武汉430062

出　　处：《中国油料作物学报》2011年第4期416-419,共4页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(30870451);“十一五”国家科技支撑计划(2010BAD01B05);国家农业产业技术体系专项(nycytx);农业部油料作物生物学重点开放实验室开放课题(201007);中国农业科学院科技项目(201012)

摘　　要：用溶液培养方法采集油菜根系分泌物并研究巨大芽孢杆菌A6对分泌物的趋化性。经高效液相色谱测定,发现油菜根系分泌物中含有7种有机酸和10种糖类。有机酸中琥珀酸含量最高达31.4μg/株;糖类中甘露醇含量最高达7.2μg/株。使用烧杯法和平板趋化法分别测定A6对7种有机酸、10种糖类和油菜根系的趋化性。结果表明,A6对6种有机酸有趋化性,其中对苹果酸的趋化性最强;对8种糖类也有趋化性,其中对山梨醇的趋化性最强;A6对油菜根系也有趋化性。Root exudate of oilseed rape was collected under water culture,and chemotaxis of Bacillus megaterium A6 towards the exudate was investigated.7 organic acids and 10 saccharide from the exudates were determined by high performance liquid chromatography（HPLC）.Among them,succinic acid and mannitol were the highest and their content reached 31.4μg/plant and 7.2μg/plant respectively.Beaker method and petri dish method were both used to test the chemotaxis of A6 towards root and those specific organic acid and saccharide.Results showed that A6 had chemotaxis towards rapeseed root.It also had chemotaxis towards 6 organic acid and 8 saccharide,especially towards malic acid and sorbitolum.

关 键 词：巨大芽孢杆菌 油菜 有机酸 糖类 趋化性 

分 类 号：Q939.9[生物学—微生物学] S565.4[农业科学—作物学]