10-羟基喜树碱的聚赖氨酸/海藻酸钠微胶囊的制备及其体外释药特性研究  

Preparation and in vitro release of microcapsules for controlled release of 10-hydroxycamptothecin-loaded polylysine and alginate

作　　者：李思阳[1] 郑健[1] 骆沙曼[1] 王洋[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心东北油田盐碱植被恢复与重建教育部重点实验室,黑龙江哈尔滨150040

出　　处：《中草药》2011年第9期1724-1727,共4页Chinese Traditional and Herbal Drugs

基　　金："948"计划资助项目(2011-4-16);黑龙江省留学归国基金资助项目(LC201004)

摘　　要：目的制备10-羟基喜树碱(HCPT)缓释微胶囊,测定其包封率,并考察其体外释药特性。方法以生物相容性良好的聚赖氨酸和海藻酸钠为囊壁材料,HCPT药物微粒为囊芯,采用静电吸引层层纳米自组装技术(LBL法)制备HCPT微胶囊。结果 LBL法制备的HCPT微胶囊用扫描电子显微镜和激光共聚焦显微镜观察具有明显的核-壳结构;HCPT微胶囊的包封率较高,达到(68.41±0.72)%;随着包裹层数的增加,HCPT微胶囊在体外释放速率降低。结论用LBL法制备的HCPT微胶囊具有较高的包封率和缓释特性,具有一定的应用前景。Objective To prepare microcapsules for controlled release of 10-hydroxycamptothecin（HCPT）,and determine its encapsulation efficiency and drug release profile in vitro.Methods The microcapsules loaded with HCPT were prepared using a novel coating technology based on the layer-by-layer（LBL） deposition of oppositely charged polylysine（PLL） and alginate（ALG） polyelectrolytes with good biocompatibility onto microcrystal templates.Results The microcapsules fabricated by this method had visible core-shell structure observed by scanning electron microscopy（SEM） and confocal laser scanning microscopy（CLSM）;The entrapment efficiency of the microcapsules was as high as（68.41 ± 0.72）%;The release rate of HCPT decreased as the bilayers increased in vitro.Conclusion The microcapsules of HCPT prepared by LBL have higher entrapment efficiency and are characterized with controlled drug release,which has potential for future application.

关 键 词：10-羟基喜树碱 体外释放 层层自组装 微胶囊 缓释 

分 类 号：R283.6[医药卫生—中药学]