含CpG寡脱氧核苷酸增强人肺癌细胞株A549放射敏感性的研究  

CpG oligodeoxynucleotide enhances radiosensitivity of human lung cancer cell line A549

作　　者：颜伟[1] 杨春敏[1] 贾敏[1] 李静[1] 唐和兰[1] 杜斌[1] 韩全利[1] 孙梯业 史蕊 周红[3] 

机构地区：[1]解放军空军总医院干部病房,北京100142 [2]解放军第二炮兵总医院肝胆胃肠病研究所 [3]第三军医大学药学院药理学教研室

出　　处：《西南国防医药》2011年第9期933-935,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探讨含CpG基序的寡脱氧核苷酸对人肺癌细胞株A549放射敏感性的影响。方法分别用不同浓度CpG ODN处理A549细胞,经β射线照射后,检测细胞增殖和集落形成情况,ELISA法检测细胞TNF-α、IL-12和INF-γ的分泌水平,G riess检测细胞NO的含量。结果 CpG ODN抑制A549细胞的增殖,并呈量效关系。CpG ODN(10μg/m l)联合β射线照射,抑制A549细胞的增殖作用最强,且增加了TNF-α、IL-12、INF-γ和NO的分泌。结论 CpG ODN对A549细胞有明显的放射增敏作用,其机制可能与CpG ODN抑制A549细胞增殖、增强TNF-α、IL-12、INF-γ和NO的分泌有关。Objective To investigate the influence of oligodeoxynucleotide containing CpG motif（CpG ODN） on the radiosensitivity of human lung cancer cell line A549.Methods A549 cells were exposed to CpG ODN at different concentration（1,3,10,30 μg/ml） and β-ray irradiation（5 Gy）.The cell proliferation and colony formation were measured afterward.The secretion levels of TNF-α,IL-12 and INF-γ were detected by ELISA method.The NO level was detected by Griess method.Results CpG ODN inhibited the proliferation of A549 cells,and presented a dose-effect relationship.CpG ODN（10 μg/ml） combined with β-ray irradiation had the best effects on inhibiting the proliferation of cells,and it increased the secretion of TNF-α,IL-12,INF-γ and NO.Conclusion CpG ODN has an obvious radiosensitizing effect on A549 cells,which may be related to its inhibition of A549 cell proliferation and enhancement of the secretion of TNF-α,IL-12,INF-γ and NO.

关 键 词：肺癌 CPGODN A549 放射敏感性 

分 类 号：R734.2[医药卫生—肿瘤]