甜樱桃MADS box基因的克隆与表达分析  

Cloning and Expression Analysis of MADS box Gene in Sweet Cherry(Prunus avium)

作　　者：林苗苗[1] 赵长竹[1] 姜建福[1] 顾红[1] 陈锦永[1] 方金豹[1] 

机构地区：[1]中国农业科学院郑州果树研究所,中国农业科学院果树生长发育与品质控制重点开放实验室,郑州450009

出　　处：《园艺学报》2011年第8期1462-1468,共7页Acta Horticulturae Sinica

基　　金：‘十一五’国家科技支撑计划项目(2006BAD07B06-10);中国农业科学院果树生长发育与品质控制重点开放实验室项目

摘　　要：以甜樱桃'拉宾斯'(Prunus avium L.'Lapins')为试材,采用RT-PCR结合RACE技术,克隆获得1个与花发育相关的MADS box基因,命名为PaMADS3,其在GenBank中的登录号为HQ229605。PaMADS3基因全长1095bp,包含1个723bp的开放阅读框,推断其编码240个氨基酸。序列分析表明:PaMADS3蛋白与拟南芥中的SEP蛋白高度同源。组织特异性表达显示PaMADS3基因在花瓣、雄蕊、心皮中表达。实时定量RT-PCR分析表明,花芽露绿期的离体枝条经过15和25℃处理后,其雌蕊中PaMADS3基因在25℃的表达量高于在15℃的表达量。A MADS box gene was isolated from the floral bud of sweet cherry（Prunus avium L.＇Lapins＇）by using RT-PCR and RACE approaches,the gene was named PaMADS3（The accession number in GenBank：HQ229605）.The full length cDNA of PaMADS3 is 1 095 bp,with an ORF of 723 bp encoding 240 putative amino acid residues.Sequence analysis indicated that PaMADS3 shared highly homology with Arabidopsis SEP gene.Tissue specific analysis showed that PaMADS3 expressed in petal,stamen,and carpel.Real-time RT-PCR revealed that when the shoots were treated with 15℃ and 25℃,respectively,the expression of PaMADS3 was increased in carpel under the temperature of 25℃.

关 键 词：甜樱桃 花发育 MADS BOX 克隆 表达 

分 类 号：S662.5[农业科学—果树学]