miRNA-122a靶基因预测及生物信息学分析  

Bioinformatic Analysis and Prediction of miRNA-122a Target Genes

作　　者：蒋永容[1] 陈川[1] 张志敏[1] 郑继军[1] 许文[1] 罗美林[1] 戴楠[1] 李梦侠[1] 杨宇馨[1] 李增鹏[1] 王东[1] 王阁[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肿瘤中心,重庆市400042

出　　处：《中国肿瘤临床》2011年第16期931-934,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金面上项目(编号:30973457)和国家自然科学基金青年科学基金项目(编号:30901764)资助~~

摘　　要：目的:利用基因芯片技术分析肝癌HepG2细胞和正常肝上皮LO2细胞中miRNA的表达,并对HepG2细胞中低表达的miRNA-122a进行靶基因预测及相关生物信息学分析,为以miRNA-122a为靶点的基因治疗提供理论和实验基础。方法:利用基因芯片技术检测HepG2细胞和LO2细胞中miRNA-122a表达水平,通过生物信息学预测miRNA-122a的靶基因,并对其靶基因进行功能富集分析(GO-analysis)、信号转导通路富集分析(Pathway-analysis)和蛋白质相互作用网络分析。结果:与LO2细胞比较,miRNA-122a在HepG2细胞中呈低表达。miRNA-122a预测靶基因有1104个,其靶基因集合功能分别富集于碳水化合物生物合成、核苷酸代谢、细胞因子受体结合、细胞周期等生物学过程(P<0.001);信号转导通路显著富集于JAK-STAT信号通路、Wnt信号通路、MAPK信号通路、ErbB信号通路、细胞周期等信号转导通路(P<0.001)。结论:miRNA-122a在HepG2细胞中呈现低表达,miRNA-122a预测靶基因集合显著富集在与肿瘤发生相关的信号通路中。Objective： The present study aimed to investigate miRNA expression patterns in hepatocellular carcinoma t nep^Jz and normol liver epithelial （LO2） cell lines. Another aim was to bioinformatically analyze as well as predict the target genes of miR-122a to provide both theoretical and experimental basis for gene therapy. Methods： The expression levels of miRNA-122a in HepG2 and LO2 cells were detected using the gene chip technology. The bioinformatic analysis of the target genes of miRNA-122a involved enrichment （ gene ontology ）, signal transduction pathway enrichment, and protein interaction network analyses. Results： miRNA-122a expression significantly decreased in HepG2 cells, compared with LO2 cells. The number of miRNA-122a target genes was 1104. The functions of these target genes were enriched in carbohydrate biosynthesis, nucleotide metabolism, cytokine receptor binding, cell cycle, and other biological processes （ P 〈 0.001 ）. The JAK-STAT signaling, Writ signaling, MAPK signaling, ErbB signaling, and cell cycle signal transduction pathways were significantly enriched （ P 〈 0.001 ）. Conclusion： miRNA-122a expression significant- ly decreased in HepG2 cells. Some of the predicted target genes of miRNA-122a were significantly enriched in tumor related with sig- naling pathways.

关 键 词：miRNA-122a 基因芯片 靶基因 生物信息学 

分 类 号：R735.7[医药卫生—肿瘤]