昆虫病原线虫共生菌对玉米弯孢菌的抑菌活性及其抗逆性  

Antibiotic Activities of the Entomopathogenic Nematodes Bacteria to Curvularia lunata Waker and the Adversity Resistance

作　　者：王欢[1,2] 刘彦群[2] 董辉[3] 钱海涛[3] 李天来[1] 丛斌[3] 郑婧[2] 谢迪[2] 高眃晞 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161 [2]沈阳农业大学生物科学技术学院,沈阳110161 [3]沈阳农业大学植物保护学院,沈阳110161

出　　处：《沈阳农业大学学报》2011年第3期325-328,共4页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金项目(30800803);农业部公益性行业项目(200803001);辽宁省自然科学基金项目(20022084);沈阳农业大学青年教师科研基金资助项目(2005002)

摘　　要：利用从国内采集土样中收集到的23个品系昆虫病原线虫和5个本室保存的线虫品系分离共生菌,通过对共生菌菌株发酵液和初提物抑菌活性的测定,得到1株对玉米弯孢菌具有良好抑菌活性的共生菌菌株SY5,抑菌圈平均直径分别为50.00mm和33.34mm,并对抑菌活性强的菌株发酵液的抗逆性进行了初步探索,结果表明,共生菌菌株SY5在50℃下处理60 min和100℃高温下10 min,其抑菌活性依旧很高;18W紫外线照射120 min对共生菌SY5的抑菌活性的影响不明显;常温保存150d,抑菌活性下降。Twenty-eight strains of symbiotic bacteria were isolated from 5 species saved in the laboratory and 23 entomopathogenicnematodes in the soil samples collected from different regions of China. The antibiotic activities of 28 strains symbiotic bacteria were examined by bioassays. Bioassay results showed that the broth and the culture crude extract of SY5 had highly antibiotic activity to Curvularia lunata Waker,the diameter of antibiotic circle were 50.00ram and 34.34mm.The adversity resistance showed that the highly virulent strain SY5 had antibiotic activity to Curvularia lunata Waker at 50℃ 60 min and 100 ℃ 10 min. The culture broth waved by ultraviolet rays from 10 min to 120 min, the antibiotic activity was still stable. The antibiotic activity of the broth decreased at room temperature for 150 days.

关 键 词：昆虫病原线虫共生菌 玉米弯孢菌 抑菌活性 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]