卒中后抑郁模型大鼠杏仁核神经元凋亡增加  

Apoptosis increased in amygdala neurons of post-stroke depression model rats

作　　者：刘昊[1] 王海涛[2] 徐爱军[2] 李世英[1] 张晋霞[1] 张蕊[1] 

机构地区：[1]河北联合大学附属医院神经内科,河北唐山063000 [2]河北联合大学基础医学院组织胚胎学教研室,河北唐山063000

出　　处：《中风与神经疾病杂志》2011年第8期685-687,共3页Journal of Apoplexy and Nervous Diseases

基　　金：河北省卫生厅医学科学研究重点课题(No.20100469)

摘　　要：目的探讨卒中后抑郁(post-stroke depression,PSD)模型大鼠杏仁核神经元凋亡现象。方法将成年健康雄性SD大鼠随机分为对照组(15只)和模型组(15只)。采用颈总动脉线栓再灌注法制备局灶性脑缺血大鼠模型,结合孤养和慢性不可预见的温和性应激方法制备PSD大鼠模型。采用糖水实验、开场实验和Morris水迷宫检测行为学改变,TUNEL和流式细胞术检测杏仁核神经元凋亡和凋亡率。结果模型组大鼠行为学发生明显改变。对照组和模型组凋亡细胞阳性率分别为3.91%±0.49%和25.04%±6.32%,凋亡率分别为3.50%±0.5%和18.75%±2.95%,差异均有统计学意义(P<0.01)。结论 PSD模型大鼠杏仁核存在明显的神经元凋亡,这可能是PSD患者杏仁核体积异常的原因之一。Objective To observe the neuronal apoptosis in amygdala of post-stroke depression（PSD） model rats.Methods Male Wistar rats were randomly divided into control group and model group.The model of focal cerebral ischemia was set up by thread occlusion through arteria carotis interna and reperfusion.Then the rats were raised alone and given chronic unpredictable modest stress to establish the PSD model.The behavior was examined using sucrose preference test,open-filed test and Morris water maze.TUNEL-staining and double-labeled flow cytometry（FCM） were employed for the detection and quantification of the apoptotic cells in the amygdala.Results The consumption of sucrose and erect quantity of model group were lower than those of control group（P〈0.05）.The percentage of TUNEL-positive cell and the apoptotic cell rate of model group were higher than those of control group respectively（P〈0.01）.Conclusion Apoptosis is increased in amygdala neurons of PSD model rats,and might be one of reasons that induced the amygdala become smaller in PSD.

关 键 词：卒中后抑郁 杏仁核 凋亡 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]