延迟注射Z-VAD对大鼠局灶性脑缺血神经细胞凋亡及炎症的影响  

The effect of apoptosis and inflammation in rats with transient middle cerebral artery occlusion by delayed Z-VAD-fmk administration

作　　者：吴晓冬[1] 王丽娜[2] 薄立华[1] 刘勇 朱辉[4] 

机构地区：[1]吉林大学中日联谊医院中心研究室,吉林长春130033 [2]吉林大学白求恩第一医院儿科,吉林长春130021 [3]美国纽约州立脑发育不全研究所 [4]吉林大学白求恩第一医院神经内科,吉林长春130021

出　　处：《中风与神经疾病杂志》2011年第8期718-720,共3页Journal of Apoplexy and Nervous Diseases

基　　金：吉林大学科学前沿与交叉学科创新项目(200903058);吉林省科技厅资助项目(20100729)

摘　　要：目的探讨延迟注射Caspase抑制剂Z-VAD-fmk对大鼠局灶性脑缺血神经细胞凋亡和炎症的影响。方法采用大鼠大脑中动脉局灶性脑梗死动物模型(MCAo),60只健康Wistar大鼠分为Z-VAD-fmk治疗组和生理盐水对照组。脑血流再通30min后侧脑室注射Z-VAD-fmk(70μg/kg),对照组注射同体积的生理盐水,分别在MCAo术后第1天、第4天、第7天处死各组动物,采用TUNEL染色检测凋亡细胞,ED-1染色检测巨噬细胞和活化的小胶质细胞。结果 TUNEL染色阳性细胞在术后第1天开始Z-VAD-fmk治疗组比对照组明显减少(P<0.05),ED-1染色阳性细胞从第4天开始有明显下降(P<0.05)。结论延迟注射Z-VAD-fmk可以减少细胞凋亡,同时也减少炎症的发生。Objective To identify the neuroprotective effect by delayed Z-VAD-fmk administration in rats with transient middle cerebral artery occlusion.Methods 60 transient MCAO rats were divided randomly into saline control group and Z-VAD-fmk treatment group.The sections that at the level of optic chiasma was selected for TUNEL and ED-1 immunohistochemistry staining on 1d,4d and 7d after the operation.Results The ED-1 positive cells number was significant different between control and treatment group both on 4d and 7d.The TUNEL positive cells were reduced significantly in the treatment group on 1d,4d and 7d.Conclusions Delayed Z-VAD-fmk administration in rats with transient middle cerebral artery occlusion showes neuroprotective effect by suppressing the inflammation and the apoptosis.

关 键 词：脑缺血 Z-VAD-FMK 凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]