PTP1B基因敲除小鼠的繁育及基因鉴定  

Propagation of PTP1B Gene Knockout Mice and Its Genotype Identification

作　　者：何凤霞[1] 徐莹[1] 徐琛[1] 

机构地区：[1]南京大学生命科学学院医药生物技术国家重点实验室,江苏南京210093

出　　处：《安徽农业科学》2011年第24期14907-14908,14977,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(81072682)

摘　　要：[目的]探讨PTP1B基因敲除小鼠的繁殖和鉴定方法,为进一步研究蛋白酪氨酸磷酸酶的功能奠定基础。[方法]将所引进的杂合子小鼠进行饲养并繁殖,繁殖成功后其子代中将会出现野生型、杂合子以及纯合子3种基因型。提取子鼠鼠尾基因组DNA,利用PCR方法扩增野生型和敲除基因片段,并根据基因片段长度来判断小鼠的基因型。[结果]PTP1B基因杂合子小鼠互交繁殖结果基本符合孟德尔遗传规律,PTP1B基因缺失纯合子小鼠无繁殖能力。[结论]利用PCR方法能够准确鉴定PTP1B基因突变小鼠基因型。[Objective] The research aimed to discuss the propagation and identification methods of PTP1B gene knockout mice and lay the foundation for further study on the function of protein tyrosine phosphatase.[Method] The introduced heterozygous mice were fed and propagated.After successful propagation,there were three genotypes（wild-type,heterozygote and homozygote） in the offspring.The genomic DNA was extracted from the tail tissue of each genotype mice and the wild type and knockout-gene fragment were amplified by using PCR method.And the genotypes of mice were judged according to the length of gene fragment.[Result] The intercrossing results of heterozygous mice with PTP1B gene were basically accordant with Mendelian inheritance laws and PTP1B gene-loss homozygous mice had no propagation ability.[Conclusion] The genotype of PTP1B gene mutant mice could be accurately identified by using PCR method.

关 键 词：PTP1B 基因敲除 基因型鉴定 

分 类 号：S814.1[农业科学—畜牧学]