黑皮果蔗花叶病茎尖脱毒技术研究  

Shoot-tip Virus-free Techniques of Black Sugarcane Mosaic Disease

作　　者：唐红琴[1] 淡明[1] 李松[1] 余坤兴[1] 黄诚华[1] 刘丽敏[1] 刘红坚[1] 卢曼曼[1] 

机构地区：[1]广西壮族自治区甘蔗研究所,广西南宁530007

出　　处：《安徽农业科学》2011年第25期15345-15346,共2页Journal of Anhui Agricultural Sciences

基　　金：广西甘蔗研究所基本科研业务专项(G2009006;G2010013);现代农业产业技术体系建设专项(nycytx-024-01-07)

摘　　要：[目的]利用果蔗热带种(Badila)茎尖培养进行甘蔗花叶病的脱毒研究。[方法]采用黑皮果蔗茎尖作为外植体进行了组织培养,并在生根阶段和假植阶段进行了甘蔗花叶病毒的分子检测。[结果]初代培养基为MS+0.50 mg/L6-BA+0.01 mg/LNAA+30 g/L蔗糖,继代培养基为MS+0.20 mg/L6-BA+0.01 mg/LNAA+30 g/L蔗糖。甘蔗花叶病毒检测结果为阴性。[结论]利用热处理结合茎尖培养能够有效脱除花叶病毒。[Objective] The detoxification of sugarcane mosaic disease was studied by mean of sugarcane tropical species（Badila） shoot-tip culture.[Method] The shoot tip of black sugarcane was used as explants for tissue culture,and the sugarcane mosaic virus in rooting phase and single plant planting stage was detected at molecular level.[Result] The early generations medium was MS＋0.05 mg/L 6-BA＋0.01 mg/L NAA＋30 g/L sucrose,and the subculture medium was MS＋0.20 mg/L BA＋0.01 mg/L NAA＋30 g/L sucrose.The molecular detection of sugarcane mosaic virus in rooting phase and single plant planting stage showed negative.[Conclusion] Shoot-tip culture combined with the use of heat treatment can be effective removal mosaic virus.

关 键 词：黑皮果蔗 甘蔗花叶病 茎尖培养 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]