生姜对全脑缺血再灌注大鼠脑组织炎性细胞因子和黏附分子含量的影响  

Effects of aqueous extract from ginger on inflammatory cytokine and adhesion molecule in brain tissue of cerebral ischemia reperfusion in rats in vivo

作　　者：王军[1] 张红霞[2] 贾士奇[1] 王玉升[1] 

机构地区：[1]河南省中医药研究院药理实验室,郑州450004 [2]信阳职业技术学院药理教研室,河南信阳464001

出　　处：《医药论坛杂志》2011年第15期12-14,18,共4页Journal of Medical Forum

基　　金：河南省属科研单位社会公益项目预研专项资金(0641130503)

摘　　要：目的观察生姜对全脑缺血再灌注大鼠脑组织炎性细胞因子和黏附分子含量的影响。方法SD大鼠随机分为假手术组、模型组、尼莫地平组（30mg/kg）和生姜水提物组（100mg／kg），Pulslnelli四动脉阻断法造成SD大鼠全脑缺血再灌流损伤模型，分别于手术前1日、术前1h和再灌注前30minig给药，共3次。酶联免疫吸附法（ELISA）测定脑组织白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF—α）和细胞间黏附分子-1（ICAM-1）、血管细胞间粘附分子-1（VCAM-1）、内皮细胞选择素（E—seletin）、血小板选择素（P—seletin）含量。结果与假手术组比较，模型对照组大鼠脑组织TNF-α、ICAM-1、P—selectin显著升高（P〈0．05）；其他炎性细胞因子（IL-1β、IL-6、IL-8）和黏附分子（VCAM-1、E—seleetin）均有不同程度的升高，但无统计学意义（P〈0．05）。生姜水提物能明显降低大鼠脑组织TNF-α、ICAM-1、P—selectin含量（P〈0．05～0．01），对其他炎性细胞因子（IL-1β、IL-6、IL-8）和黏附分子（ICAM-1、VCAM-1、E～selectin）均有不同程度的降低作用，但无统计学意义（P〈0．05）。结论炎性细胞因子与黏附分子及由此介导的炎症级联反应参与缺血再灌注性脑损伤的病理过程，生姜治疗脑缺血再灌注损伤的机制之一与降低炎性细胞因子与黏附分子含量、抑制炎症级联反应有关。Objective To investigate the effects of aqueous extract from ginger on inflammatory cytokines and adhesion molecules in brain tissue after cerebral ischemia reperfusion （CIR） in rats. Methods Male SD rats were randomly divided into 6 groups as following： sham -operation group, model control group, nimodipine group and group of aqueous extract from ginger. CIR model in rats were reproduced by Pulsinelli＇s ＂Four Vessel Occlusion＂ method. The contents of cytokine- 1β（IL- 1β）, cytokine - 6 （ IL - 6）, cytokine - 8 （ IL - 8 ） and tummor necrosis factor - α （ TNF - α ） and inter- cellular adhesoin molecule - 1 （ ICAM - 1 ） , vascular cell adhesion molecule - 1 （ VCAM - 1 ） , endo- thelium- selectin（ E- selectin） and platelet- selectin （P- selectin） in brain tissue were detected by enzyme - linked immunoadsordent assay（ELISA）. Results Compared with sham- operation group, the contents of TNF -α ,ICAM - 1 and P - selectin in brain tissue of model group increased significantly （P 〈 0.05 ） While compared with model group, aqueous extract of ginger could decrease the contents of TNF -α, ICAM - 1 and P - selectin in brain tissue（ P 〈 0. 05 -0. 01 ）, but no significant difference to the content of IL- 1β,IL-6, IL- 8, ICAM- 1, VCAM -1 and E -selectin, compared with model control group. Conclusions The therapeutic action of ginger on cerebral ischemia reperfusion can be realized by decreasing the in- flammatory response induced by cytokines and adhesion molecules.

关 键 词：生姜 脑缺血 细胞因子 黏附分子 大鼠 

分 类 号：R-332[医药卫生]