中国南海新型α-芋螺毒素Im1.6的克隆及合成  

Cloning and synthesis of a novel alpha conotoxin sequence Im1.6 from South China Sea

作　　者：刘娜[1,2] 刘珠果[2] 孙婷[2] 崔志芳[1] 戴秋云[2] 

机构地区：[1]山东科技大学化学与环境工程学院,山东青岛266510 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《军事医学》2011年第8期570-572,586,共4页Military Medical Sciences

基　　金：国家973计划项目(2010CB529802);海洋863项目(2011AA090701);国家自然科学基金项目(9071028)

摘　　要：目的从中国南海堂皇芋螺(Conus imperialis)中克隆出新的芋螺毒素序列并用化学方法合成该毒素。方法根据A超家族保守的信号肽序列设计引物,采用cDNA的3'端快速扩增方法(3'RACE),从芋螺毒腺管中克隆出新的毒素基因。固相法合成线性多肽,折叠形成目标产物,用两步氧化折叠法测定多肽的二硫键连接方式。结果发现一种新的α-芋螺毒素cDNA序列Im1.6,其编码的成熟肽序列为GCCDIPDCYNKNREQC-NH2,二硫键连接方式为"C1-C3,C2-C4"。结论 Im1.6是一种新的α4/7型芋螺毒素,其氨基酸序列与报道的α-芋螺毒素显著不同。Objective To clone a new conotoxin from Conus imperialis derived from the South China Sea and synthesize the peptide. Methods Alpha-conotoxin was cloned in ConuS venom by 3＇RACE based on the conserved signal peptide se- quence of A-super-family. The conotoxin was synthesized using solid-phase method and folded into the target product whose disulfide bridge connection was further determined by two-step oxidative folding. Results A novel conotoxin designated as Iml. 6 （ GCCDIPDCYNKNREQC-NH2 ） was obtained, whose disulfide connectivity was ＂ C1-C3, C2-C4＂. Conclusion Iml. 6 belongs toα4/7 subfamily and is remarkably different from the reoorted α-conotoxins.

关 键 词：芋螺毒素类 4/7型 克隆 合成 二硫键骨架 

分 类 号：R914[医药卫生—药物化学]