臧红T-Fenton体系荧光光谱法测定黄山贡菊/金菊的抗氧化活性  

Determination of Antioxidant Activity of Huangshan Chrysanthemum/Golden Aster by Fluorometry with Safranine T-Fenton System

作　　者：徐金明[1] 陈欢[1] 

机构地区：[1]黄山学院化学系

出　　处：《光谱实验室》2011年第5期2455-2459,共5页Chinese Journal of Spectroscopy Laboratory

摘　　要：臧红T能产生特征荧光,其激发波长和发射波长分别为264nm和577nm。酸性介质中,F en ton体系(H2O2-F e2+)产生的羟自由基能氧化臧红T使其荧光猝灭,而许多花茶的提取物可部分清除溶液中的羟自由基使其荧光猝灭程度降低,据此建立了一种测定黄山贡菊和金菊的抗氧化活性的新方法。本实验采用单因素实验法,对羟自由基体系各种条件进行了优化,并根据各体系的荧光强度计算出自由基清除率分别是64.8%、50.9%。Characteristic fluorescence produced by safranine T was observed with its excitation and emission wavelength at 264nm and 577nm,respectively.Fluorescence intensity of safranine T was quenched by the hydroxyl free radical produced by Fenton system in acidic medium.However,this quenching effect can be alleviated by some extractants of flower tea,because the hydroxyl free radical can be partially cleaned.Based on this principle,a fluorometry method was developed for the determination of the antioxidant activities of Huangshan chrysanthemun and golden aster.Various conditions of hydroxyl radicals system were optimized by single-factor test,and the clearance rate of free radicals for Huangshan chrysanthemun and golden aster were 64.8% and 50.9%,respectively.

关 键 词：臧红T 荧光分析法 黄山贡菊 抗氧化活性 

分 类 号：O657.32[理学—分析化学]